The concentration of this product will vary from lot to lot and will range from 1.2 – 1.6 mL for the 25 mg package size. The exact concentration will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate::
https://www.sigmaaldrich.com/product/sigma/p3125#product-documentation
P3125
Papain from papaya latex
buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein
Synonym(s):
Papainase
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25 MG
$79.80
100 MG
$144.00
250 MG
$385.00
500 MG
$738.00
1 G
$1,340.00
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42030311
NACRES:
NA.54
MDL number:
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Product Name
Papain from papaya latex, buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein
form
buffered aqueous suspension
quality
2× Crystallized
specific activity
≥16 units/mg protein
mol wt
23.4 kDa
storage temp.
2-8°C
Quality Level
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Related Categories
1 of 4
This Item | P4762 | P5306 | P3375 |
|---|---|---|---|
| specific activity ≥16 units/mg protein | specific activity ≥10 units/mg protein | specific activity ≥8.0 units/mg protein | specific activity 1.5-10 units/mg solid |
| form buffered aqueous suspension | form lyophilized powder | form lyophilized powder | form crude powder |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| quality 2× Crystallized | quality - | quality - | quality - |
Application
Papain from papaya latex has been used to digest:
- Muller glial cells[1]
- cartilage explants[2]
- GAG and DNA content of the tissue constructs[3]
- decellularized tissue samples[4].
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies.[5] It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain has broad specificity,[6] in cleaving peptide bonds of basic amino acids, leucinehydrolyzes esters and amides.[7] Papain digests many protein substrates extensively than pancreatic proteases.[8] Papain is commonly used in cell isolation procedures since it is more efficient and less harmful compared to other proteases.[6] Papain significantly increases the yield of viable smooth muscle cells without affecting the cell′s sensitivity to stimulants.[9] It catalyzes several reactions, like hydrolysis, transferase action, specificity and acyl-enzyme intermediate.[10]
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
General description
Papain, a cysteine protease, consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group.[9]
Other Notes
One unit will hydrolyze 1.0 μmole of N-α-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 °C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
Physical form
Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix
Yu C, et al.
Biomaterials, 194, 1-13 (2019)
PTH decreases in vitro human cartilage regeneration without affecting hypertrophic differentiation
Rutgers M, et al.
Testing, 14(4), e0213483-e0213483 (2019)
Expression of non-visual opsins Opn3 and Opn5 in the developing inner retinal cells of birds. Light-responses in Muller glial cells
Rios MN, et al.
Frontiers in Cellular Neuroscience, 13, 376-376 (2019)
PAPAIN ENZYME: A DIGESTIVE AID
PATEL T
International Journal of Clinical and Biomedical Research, 52-53 (2016)
The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)
Articles
Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).
Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.
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