M5409
Mouse IgG2a Isotype Control from murine myeloma
clone UPC-10, purified immunoglobulin, buffered aqueous solution
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0.1 MG
$219.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
UPC-10, monoclonal
form
buffered aqueous solution
technique(s)
flow cytometry: suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
1 of 4
This Item | F6397 | M9035 | M1421 |
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone UPC-10, monoclonal | clone MOPC 21, monoclonal | clone MOPC 31C, monoclonal | clone TEPC 15, monoclonal |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
Application
Mouse IgG2a Isotype Control from murine myeloma has been used in:
- immunoprecipitation
- enzyme-linked immunosorbent assay (ELISA)[1]
- fluorescence microscopy
- indirect immunocytochemistry
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.
When evaluated in flow cytometry, the product did not stain human peripheral blood lymphocytes (PBLs). Anti-Mouse IgG (whole molecule), F(ab′)2 fragment FITC, Catalog Number F2883, along with 1 mg of the product was incubated with human PBLs and then evaluated by flow cytometry.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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