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LB Broth with agar (Lennox)

Name: LB Broth with agar (Lennox)
Brand: SIGMA

EZMix powder microbial growth medium

Synonym(s):

LB broth

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About This Item

NACRES:

NA.85

UNSPSC Code:

41106200

Pricing and availability is not currently available.

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grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

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Industrial Microbiology

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This Item	L2897	L7025	L7658
Sigma-Aldrich L7533 LB Broth with agar (Lennox)	Sigma-Aldrich L2897 LB Broth with agar (Lennox)	Sigma-Aldrich L7025 LB Broth with agar (Lennox)	Sigma-Aldrich L7658 LB Broth (Lennox)
suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms	suitability nonselective for Escherichia coli, nonselective for coliforms
technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable	technique(s) microbiological culture: suitable
form powder	form powder	form tablet	form powder
sterility non-sterile	sterility non-sterile	sterility non-sterile	sterility non-sterile
composition Agar, 15 g/L , Tryptone, 10 g/L , NaCl, 5 g/L , Yeast Extract, 5 g/L	composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L	composition -	composition -
application(s) agriculture food and beverages microbiology	application(s) food and beverages microbiology	application(s) food and beverages microbiology	application(s) agriculture food and beverages microbiology

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox EZMix powder with agar provides:

A budget-friendly alternative to pre-poured plates
Granulated, dust-free format for safer handling and faster mixing
Convenient small package to eliminate weighing
Easy scale-up using larger package sizes
Standard formulation

Preparation Note

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Sambrook, J., et al

Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)

Ausubel, F. M.

Current Protocols in Molecular Biology, 1, 3-3 (1994)

Quantifying protein-protein interactions in high throughput using protein domain microarrays.

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Nature protocols, 5(4), 773-790 (2010-04-03)

Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these

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Cell stem cell, 26(3), 359-376 (2020-02-29)

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Global Trade Item Number

SKU	GTIN
L7533-6X500ML	04061833965566
L7533-1KG	04061833965559

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