At M-Clarity quality level 100, Product L0259 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0259#product-documentation
L0259
Lucifer Yellow CH dilithium salt
Powder
Synonym(s):
6-Amino-2,3-dihydro-1,3-dioxo-2-hydrazinocarbonylamino-1H-benz[d,e]isoquinoline-5,8-disulfonic acid dilithium salt
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25 MG
$283.00
100 MG
$939.00
250 MG
$2,070.00
About This Item
Empirical Formula (Hill Notation):
C13H9Li2N5O9S2
CAS Number:
Molecular Weight:
457.25
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
267-047-9
MDL number:
Beilstein/REAXYS Number:
4644601
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Product Name
Lucifer Yellow CH dilithium salt, fluorescent stain
InChI
1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
InChI key
RPKCZJYDUKVMGF-UHFFFAOYSA-L
SMILES string
[Li+].[Li+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O
form
powder
color
faint orange to very dark orange
solubility
H2O: 1 mg/mL
fluorescence
λex 428 nm; λem 540 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Quality Level
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This Item | L3510 | L0144 | L6882 |
|---|---|---|---|
| color faint orange to very dark orange | color yellow to brown | color orange | color - |
| fluorescence λex 428 nm; λem 540 nm in H2O | fluorescence λex 428 nm; λem 536 nm in H2O | fluorescence λex 428 nm; λem 540 nm | fluorescence λex 328 nm; λem 532 nm in H2O |
| solubility H2O: 1 mg/mL | solubility - | solubility - | solubility H2O: soluble |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| form powder | form powder | form powder | form lyophilized powder |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
Application
A highly fluorescent dye, useful in marking nerve cells.
General description
Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. Lucifer Yellow CH dilithium salt is a fluorescent dye(1) comprising of amino, sulfo and phenyl-N-hydrazinocarbonylamino substituents on the naphthalimide chromophore. The hydrophilic nature of Lucifer yellow CH underlies its numerous applications as a stain of living cells and tissues.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Metabolic, fluorescent dye and electrical coupling between hamster oocytes and cumulus cells during meiotic maturation in vivo and in vitro.
Racowsky, C. and Satterlie, R.A.
Developmental Biology, 108, 191-202 (1985)
Mariane C Vicente et al.
Experimental neurology, 328, 113250-113250 (2020-02-24)
The locus coeruleus (LC) is a pontine nucleus important for respiratory control and central chemoreception. It is affected in Alzheimer's disease (AD) and alteration of LC cell function may account for respiratory problems observed in AD patients. In the current
Qi-You Li et al.
Experimental eye research, 202, 108305-108305 (2020-10-21)
The biosafety and efficiency of transplanting retinal pigment epithelial (RPE) cells derived from both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been evaluated in phase I and phase II clinical trials. For further large-scale application
Elisabet Miro-Casas et al.
Cardiovascular research, 83(4), 747-756 (2009-05-23)
Connexin43 is present at the inner membrane of cardiomyocyte mitochondria (mCx43), but its function remains unknown. In this study we verified the presence of mCx43 by a mass spectrometry-based proteomic approach in purified mitochondrial preparations from mouse myocardium and determined
Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer.
W W Stewart
Cell, 14(3), 741-759 (1978-07-01)
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What is the dye purity for product L0259?
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