KCQS04
KiCqStart® Probe qPCR ReadyMix™
for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonym(s):
Kicqstart® Probe qPCR ReadyMix™
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$257.00
$1,170.00
$257.00
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Product Name
KiCqStart® Probe qPCR ReadyMix™, for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
form
liquid
usage
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
feature
dNTPs included
hotstart
storage condition
protect from light
technique(s)
qPCR: suitable
color
light blue
input
purified DNA
compatibility
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
detection method
probe-based
shipped in
dry ice
storage temp.
−20°C
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This Item | KCQS05 | KCQS06 | KCQS07 |
|---|---|---|---|
| technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable |
| form liquid | form liquid | form liquid | form liquid |
| usage sufficient for 1250 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 500 reactions |
| feature dNTPs included | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition protect from light |
| color light blue | color light blue | color light blue | color light blue |
Application
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
General description
A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Legal Information
related product
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
La réaction en chaîne par polymérase (PCR) est l'une des techniques les plus couramment utilisées en biologie moléculaire. Le procédé de PCR implique trois étapes principales : la dénaturation, l'hybridation des amorces et l'élongation
Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Protocols
KiCqStart™ Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments.
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