The product includes the following components:
-5x Ligation: Contains oligonucleotides that hybridize to the PLA probes and all components needed for ligation except the Ligase.
-1x Ligase (1 unit/?L).
-1x Polymerase (10 units/?L).
-5x Amplification Red: Contains all components needed for Rolling Circle Amplification (RCA) except the Polymerase. It also contains oligonucleotide probes labeled with a fluorophore that hybridize to the RCA product.
-Primary antibodies, PLA probes, wash buffers, and mounting medium are not included in this detection kit.
DUO92008
Duolink® In Situ Detection Reagents Red
Duolink® In Situ Detection Reagents Red
Synonym(s):
in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent
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| Size/SKU | Availability | Price |
|---|---|---|
30 reactions | Check Cart for Availability | $540.00 |
100 reactions | Check Cart for Availability | $920.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
product line
Duolink®
Quality Segment
technique(s)
proximity ligation assay: suitable
fluorescence
λex 594 nm; λem 624 nm (Texas Red®, Zeiss Filter set 31)
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
Application
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Other Notes
This product is comprised of the following:
See datasheet for more information.
Not included in Detection kit:
Primary antibodies, PLA probes, wash buffers, mounting medium
- 5x Ligation - Contains oligonucleotides that hybridize to the PLA probes and all components needed for ligation except the Ligase
- 1x Ligase (1 unit/μL)
- 1x Polymerase (10 units/μL)
See datasheet for more information.
Not included in Detection kit:
Primary antibodies, PLA probes, wash buffers, mounting medium
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
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This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable |
| fluorescence λex 594 nm; λem 624 nm (Texas Red®, Zeiss Filter set 31) | fluorescence λex 554 nm; λem 576 nm (Cyanine 3; Zeiss Filter set 20) | fluorescence λex 644 nm; λem 669 nm (Cyanine 5, Zeiss Filter set 50) | fluorescence λex 495 nm; λem 527 nm (green) (FITC (Cyanine 2), Zeiss Filter set 38) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
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I was wondering in which of the PLA reagents the connector oligos are included In case it makes any difference, I’m asking about DUO92008
1 answer-
Helpful?
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Are the regents in the detection kit sold separately?
1 answer-
Unfortunately, these kit components are not sold separately. There are some available buffers and diluents available to order. For a full list of Duolink products, please see the link below:
https://www.sigmaaldrich.com/products/protein-biology/duolink-proximity-ligation-assay?country=US&language=en&cmsRoute=products&cmsRoute=protein-biology&cmsRoute=duolink-proximity-ligation-assay&page=1Helpful?
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Duolink® In Situ Detection Reagents Red (DUO92008). What reagents are included in this product?
1 answer-
Please see the link below to review the product datasheet which includes the list of components included in this kit:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/136/474/duo92008dat.pdfHelpful?
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