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MilliporeSigma

C-12375

Human Cardiac Fibroblasts (HCF)

500,000 cryopreserved cells

Synonym(s):

HCF cells

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About This Item

NACRES:
NA.81
UNSPSC Code:
41106514

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Product Name

Human Cardiac Fibroblasts (HCF), 500,000 cryopreserved cells

biological source

human heart (ventricles)

packaging

pkg of 500,000 cells

morphology

(fibroblast)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12377C-14036C-12810
Human Cardiac Fibroblasts (HCF) 500,000 cryopreserved cells

Sigma-Aldrich

C-12375

Human Cardiac Fibroblasts (HCF)

Human Cardiac Fibroblasts (HCF) 500,000 proliferating cells

Sigma-Aldrich

C-12377

Human Cardiac Fibroblasts (HCF)

Human Cardiac Fibroblasts (HCF) 1 million cells per pellet

Sigma-Aldrich

C-14036

Human Cardiac Fibroblasts (HCF)

Human Cardiac Myocytes (HCM) 500,000 cryopreserved cells

Sigma-Aldrich

C-12810

Human Cardiac Myocytes (HCM)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

biological source

human heart (ventricles)

biological source

human heart (ventricles)

biological source

human heart (ventricles)

biological source

human heart (ventricles)

relevant disease(s)

cardiovascular diseases

relevant disease(s)

cardiovascular diseases

relevant disease(s)

cardiovascular diseases

relevant disease(s)

-

morphology

(fibroblast)

morphology

(fibroblast)

morphology

(fibroblast)

morphology

(myocytes)

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

Analysis Note

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Application

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

Biochem/physiol Actions

Heart

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positive, Smooth muscle alpha-actin negative, slow muscle myosin negativeGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link

Preparation Note

Subculture Routine –Click here for more information.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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