A7607

Anti-α-Smooth Muscle Actin (ACTA2) Antibody,

Name: Anti-α-Smooth Muscle Actin (ACTA2) Antibody,
Brand: SIGMA
Price: 870 CAD

mouse monoclonal, CGA7

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200 μL

$870.00

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162107

Key Documents

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Product Name

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse, ~1.0 mg/mL, clone CGA7, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CGA7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, chicken, mouse, human, monkey

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 5-10 μg/mL using chicken gizzard total extract

isotype

IgG2b

UniProt accession no.

P62736

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

chicken ... ACTA2(423787)
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

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This Item	A2547	A2066	SAB4200689
Sigma-Aldrich A7607 Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse	Sigma-Aldrich A2547 Monoclonal Anti-Actin, α-Smooth Muscle	Sigma-Aldrich A2066 Anti-Actin antibody produced in rabbit	Sigma-Aldrich SAB4200689 Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal
Gene Information chicken ... ACTA2(423787) human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633)	Gene Information human ... ACTA2(59) mouse ... Acta2(11475) rat ... Acta2(81633)	Gene Information human ... ACTA1(58) mouse ... Acta1(11459) rat ... Acta1(29437)	Gene Information human ... ACTA2(59)
antibody form purified immunoglobulin	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
clone CGA7, monoclonal	clone 1A4, monoclonal	clone polyclonal	clone 5C5, monoclonal
species reactivity rat, chicken, mouse, human, monkey	species reactivity human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake	species reactivity wide range, vertebrates, human, slime mold, amoeba, chicken	species reactivity rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
biological source mouse	biological source mouse	biological source rabbit	biological source mouse

Application

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse has been used in:

enzyme-linked immunosorbent assay (ELISA)
immunoblotting
immunocytochemistry
immunohistochemistry

Biochem/physiol Actions

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in alpha-actin 2 (ACTA2) results in thoracic aortic aneurysm, Moyamoya disease, coronary artery disease, stroke and multisystemic smooth muscle dysfunction syndrome. It plays a functional role in the smooth muscle of non-vascular tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. Its expression is related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha actins (skeletal, cardiac, smooth), one beta actin (beta-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle).
Monoclonal Anti-Actin, Smooth Muscle, reacts with human, monkey, chicken, rat, and mouse smooth muscle actin.

Monoclonal Anti-Actin, Smooth Muscle (mouse IgG2b isotype) is derived from the hybridoma CGA7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified chicken gizzard actin. α-actin 2 (ACTA2) codes for a smooth muscle specific α-actin.

The antibody specifically recognizes α and γ smooth muscle actin.

Immunogen

partially purified chicken gizzard actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Prostate-specific G-protein-coupled receptor collaborates with loss of PTEN to promote prostate cancer progression.

M Rodriguez et al.

Oncogene, 35(9), 1153-1162 (2015-06-02)

Among frequent events in prostate cancer are loss of the tumor-suppressor phosphatase and tensin homologue (PTEN) and overexpression of prostate-specific G-protein-coupled receptor (PSGR), but the potential tumorigenic synergy between these lesions is unknown. Here, we report a new mouse model

ACTA2 mutation and postpartum hemorrhage: a case report

Cooper K and Brown S

BMC Medical Genetics, 18(1), 143-143 (2017)

Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces

Johnstone SA, et al.

Acta Biomaterialia, 13, 266-276 (2015)

Role of fibroblast growth factor signaling during proepicardium formation in the chick embryo

Torlopp A, et al.

Developmental Dynamics, 239(9), 2393-2403 (2010)

Beneficial effects of galectin-3 blockade in vascular and aortic valve alterations in an experimental pressure overload model

Ibarrola J, et al.

International Journal of Molecular Sciences, 18(8), 1664-1664 (2017)

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Questions

Anonymous

11 months ago

how long does the product remain stable when stored at -20°C?

1 answer
1. Technical Support
  
  11 months ago
  
  This product is stored at -20°C and assigned a recommended retest date of 4 years from the date of Quality Release. This date is published in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
  https://www.sigmaaldrich.com/product/sigma/a7607#product-documentation
  Please see the following link to review additional handling and storage information available in the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/123/627/a7607dat.pdf
  
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