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C7863

Sigma-Aldrich

Anti-Csk antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD01633277
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat spleen
microarray: suitable
western blot: 1:2,000 using a whole cell extract of human Jurkat cell line and a cytosolic fraction of embryonic rat brain

UniProt accession no.

P41240

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CSK(1445)
mouse ... Csk(12988)
rat ... Csk(315707)

General description

Csk is a widely expressed non-receptor tyrosine kinase that consists of a catalytic domain, as well as SH2 and SH3 domains. This kinase phosphorylates the C-terminal tail of Src-family protein tyrosine kinases (SFKs) and subsequently inhibits SFKs. As SFKs have been associated carcinogenesis, Csk may have implications in cancer therapeutics
Anti-Csk antibody is specific for human and rat Csk. The product may also react with mouse Csk. In immunoblotting, staining of Csk is specifically inhibited with the Csk immunizing peptide (rat, amino acids 433-450).

Immunogen

peptide corresponding to amino acids 433-450 at the C-terminus of rat Csk. This sequence is highly conserved in human and mouse.

Application

Anti-Csk antibody is suitable for use in western blot (1:2,000 using whole cell extracts of human Jurkat cell line and cytosolic fractions of embryonic rat brain). The product may also be used for microarray and immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections of rat spleen).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Yuh-Ping Chong et al.
Growth factors (Chur, Switzerland), 23(3), 233-244 (2005-10-26)
C-terminal Src kinase (CSK) and CSK-homologous kinase (CHK) are endogenous inhibitors of the Src-family protein tyrosine kinases (SFKs). Since constitutive activation of SFKs contributes to cancer formation and progression, to prevent excessive activation of SFKs, their activity in normal cells
D Sondhi et al.
Biochemistry, 38(34), 11147-11155 (1999-08-25)
Csk (C-terminal Src kinase) is a protein tyrosine kinase that phosphorylates Src family member C-terminal tails, resulting in downregulation of Src family members. It is composed of three principal domains: an SH3 (Src homology 3) domain, an SH2 (Src homology
A Shekhtman et al.
Journal of molecular biology, 314(1), 129-138 (2001-11-29)
Csk (C-terminal Src kinase), a protein tyrosine kinase, consisting of the Src homology 2 and 3 (SH2 and SH3) domains and a catalytic domain, phosphorylates the C-terminal tail of Src-family members, resulting in downregulation of the Src family kinase activity.

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