53399
Anti-Kaninchen IgG−Abberior® STAR 635P in Ziege hergestellte Antikörper
for STED application
Synonym(e):
Abberior® STAR 635P-Anti-Kaninchen IgG in Ziege hergestellte Antikörper
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NACRES:
NA.45
UNSPSC Code:
12352200
Technischer Dienst
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Unterstützung erhaltenTechnischer Dienst
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Unterstützung erhaltenbiological source
goat
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rabbit
concentration
~1 mg/mL
fluorescence
λex 638 nm; λem 651 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 635P was the first member of an entirely new class of fluorescent dyes introduced exclusively by Abberior: the class of phosphorylated fluorescent dye. The dye excels in its photophysical parameters: it is very bright, very stable and it enables basically background free imaging. The dye works exceptionally well with Abberior Instruments STEDYCON und Expert line microscopes and Leica STED microscopes. Abberior STAR 635P can substitute Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR 635P is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 120 000 (H2O), 130 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,21 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,40 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 651 (PBS pH 7.4), 651 (H2O), 651 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,3 (PBS pH 7.4)
Application
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Verwandter Inhalt
53399, Anti-Rabbit IgG-Abberior STAR 635P
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