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MilliporeSigma

O1875

Orcinol monohydrate

≥99% purity (HPLC), powder

Synonym(s):

3,5-Dihydroxytoluene, 5-Methylresorcinol

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About This Item

Linear Formula:
CH3C6H3-1,3-(OH)2 · H2O
CAS Number:
6153-39-5
Molecular Weight:
142.15
NACRES:
NA.47
PubChem Substance ID:
24897953
UNSPSC Code:
12171500
EC Number:
207-984-2
MDL number:
MFCD00149092
Beilstein/REAXYS Number:
1071903

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Product Name

Orcinol monohydrate, colorimetric detection reagent

InChI key

NBKPNAMTHBIMLA-UHFFFAOYSA-N

InChI

1S/C7H8O2.H2O/c1-5-2-6(8)4-7(9)3-5;/h2-4,8-9H,1H3;1H2

SMILES string

O.Cc1cc(O)cc(O)c1

biological source

synthetic

assay

≥99% (HPLC)

form

powder

technique(s)

titration: suitable

color

white to light brown

bp

290 °C (lit.)

mp

56-58 °C (lit.)

solubility

H2O: 50 mg/mL

density

1.290 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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1 of 4

This Item
H46805D4645447420
Orcinol monohydrate colorimetric detection reagent

Sigma-Aldrich

O1875

Orcinol monohydrate

2-Hydroxy-1,4-naphthoquinone 97%

Sigma-Aldrich

H46805

2-Hydroxy-1,4-naphthoquinone

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate used for peroxide measurement

Sigma-Aldrich

D4645

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate

Orcinol 97%

Sigma-Aldrich

447420

Orcinol

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

color

white to light brown

color

faint yellow to dark yellow, and Faint Orange to Dark Orange

color

white to off-white

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

solubility

H2O: 50 mg/mL

solubility

-

solubility

H2O: 50 mg/mL

solubility

-

form

powder

form

powder

form

powder

form

solid

assay

≥99% (HPLC)

assay

97%

assay

≥98% (TLC)

assay

97%

Application

Orcinol monohydrate has been used to prepare Bial′s reagent for Bial′s orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.[1]

[1]

[1]

General description

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes.[2] Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial′s reagent for the determination of RNA, DNA, and hexoses.[3]

flash_point_f

Not applicable

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000505727
0000505727
0000505728
0000505729
0000505729

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Natural products as fungicide and their role in crop protection. et al.
Natural Antimicrobials for the Minimal Processing of Foods, 131-131 (2020)
Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples.
Patterson Jennifer and Cameron Mura
Journal of Visualized Experiments (2013)
A modified orcinol reaction for RNA determination.
M Kamali et al.
Clinical chemistry, 15(5), 390-392 (1969-05-01)
Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells.
Grube BJ
The Journal of Biological Chemistry, 269, 8477-8477 (1994)
A simultaneous estimation method of DNA and RNA by the orcinol reaction and a study on the reaction mechanism.
Y Endo
Journal of biochemistry, 67(5), 629-633 (1970-05-01)
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