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A3648

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane

≥98%

Synonym(s):

APTS, 3-Triethoxysilylpropylamine, APTES

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$404.00

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100 ML
$114.00
500 ML
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About This Item

Linear Formula:
H2N(CH2)3Si(OC2H5)3
CAS Number:
919-30-2
Molecular Weight:
221.37
Beilstein:
1754988
EC Number:
213-048-4
MDL number:
MFCD00008207
UNSPSC Code:
12171500
PubChem Substance ID:
24890794
NACRES:
NA.47

$114.00

In StockDetails

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Quality Level

200

Assay

≥98%

form

liquid

pH

11 (20 °C, 20 g/L)

bp

217 °C/760 mmHg (lit.)

mp

220-222 °C

density

0.946 g/mL at 25 °C (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CCO[Si](CCCN)(OCC)OCC

InChI

1S/C9H23NO3Si/c1-4-11-14(12-5-2,13-6-3)9-7-8-10/h4-10H2,1-3H3

InChI key

WYTZZXDRDKSJID-UHFFFAOYSA-N

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Application

  • (3-Aminopropyl)triethoxysilane has been used for the preparation of cadherin surfaces on glass slides.[1]
  • It has been used for the preparation of RGD (Arg-Gly-Asp)-treated silicone membranes.[2]
  • It has been used for the preparation of the avidin–biotin coverslips.[3]

Biochem/physiol Actions

(3-Aminopropyl)triethoxysilane forms aminopropyl derivative of glass, an adsorbent for affinity chromatography. It is used to prepare positively charged slides suitable for use with various immunohistochemical and in situ hybridization procedures.[4]

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H314,H317

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

199.4 °F - closed cup

Flash Point(C)

93 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF5027V
WXBF4299V
WXBF3409V
WXBF3660V
WXBF2194V

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Apoptosis and survival of osteoblast-like cells are regulated by surface attachment.
Grigoriou V, et al.
The Journal of Biological Chemistry, 280, 1733-1739 (2005)
Real-time observation of a single DNA digestion by lambda exonuclease under a fluorescence microscope field.
Matsuura S, et al.
Nucleic Acids Research, 29, E79-E79 (2001)
Isabelle Bonnet et al.
Nucleic acids research, 36(12), 4118-4127 (2008-06-12)
The restriction endonuclease EcoRV can rapidly locate a short recognition site within long non-cognate DNA using 'facilitated diffusion'. This process has long been attributed to a sliding mechanism, in which the enzyme first binds to the DNA via nonspecific interaction
Sri Ram Krishna Vedula et al.
Nature communications, 6, 6111-6111 (2015-01-23)
The closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive
H Delanoë-Ayari et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(8), 2229-2234 (2004-02-26)
Physicists have studied the aggregation of adhesive proteins, giving a central role to the elastic properties of membranes, whereas cell biologists have put the emphasis on the cytoskeleton. However, there is a dramatic lack of experimental studies probing both contributions
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Questions

1–6 of 6 Questions  

  1. Can I buy glass slides already treated with Product A3648, (3-Aminopropyl)triethoxysilane?

    1 answer

    1. Yes. Silane Prep slides, Product No. S4651, are produced using A3648. These slides have an expiration period of 24 months.

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  2. How does Product A3648, (3-Aminopropyl)triethoxysilane, compare to poly-L-lysine for binding tissue to glass surfaces?

    1 answer

    1. This product has been cited as being a better tissue adhesive than poly-L-lysine. Maddox PH, Jenkins D., 3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion. J Clin Pathol., 40(10):1256-7 (1987).

      Helpful?

  3. What is the concentration of the (3-Aminopropyl)triethoxysilane, commonly known as APTES?

    1 answer

    1. The (3-Aminopropyl)triethoxysilane, which is a neat liquid, is 4.3 M. This is based on a density of 0.946 g/mL and a molecular weight of 221.37.

      Helpful?

  4. Can Product A3648, (3-Aminopropyl)triethoxysilane, be used to coat controlled pore glass?

    1 answer

    1. To coat controlled-pore glass beads for affinity chromatography, the following method is recommended:1. Treat 1 g of clean beads with 20 mL of a 10% aqueous A3648 (pH 3 to 4) at 75 °C for 2 hours.2. Filter the beads to remove the solution and dry overnight in a 115 °C oven. See: J. Dairy Sci., 73, 2720-2730 (1990).

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. Can the coating made from product A3648, (3-Aminopropyl)triethoxysilane, be removed?

    1 answer

    1. For Product A3648, (3-Aminopropyl)triethoxysilane, the siliconized coating can be removed by an overnight treatment with 10-20% (w/v) aqueous or alcoholic potassium hydroxide or sodium hydroxide solutions. It may also be possible to remove the coating by physical abrasion, i.e., scrubbing or vigorously brushing the glass surface. Autoclaving will not destroy the coating.

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