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6.25 ML
$289.00
$289.00
Estimated to ship onDecember 22, 2025Details
About This Item
NACRES:
NA.54
UNSPSC Code:
41106300
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Product Name
KAPA Taq ReadyMix, 2 ×, suitable for PCR
usage
50 μL sufficient for 250 reactions
shelf life
≤18 mo.
feature
dNTPs included
hotstart: no
packaging
pkg of 6.25 mL
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
Quality Level
1 of 4
This Item | P4600 | TXHSRMKB | TAQDKB |
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable |
| usage 50 μL sufficient for 250 reactions | usage sufficient for 100 reactions | usage - | usage - |
| shelf life ≤18 mo. | shelf life - | shelf life - | shelf life ≤18 mo. |
| feature dNTPs included | feature dNTPs included, hotstart: no | feature dNTPs included, hotstart | feature dNTPs included: no, hotstart: no |
| packaging pkg of 6.25 mL | packaging - | packaging pkg of 1.25 mL (KK3604), pkg of 6.25 mL (KK3605) | packaging pkg of 250 U (KK1020), pkg of 2500 U (BK1004), pkg of 500 U (KK1022), pkg of 5000 U (BK1006) |
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename Roche |
Analysis Note
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq ReadyMixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Application
Biochem/physiol Actions
KAPA Taq and KAPA Taq HotStart® DNA Polymerase elicit 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′-5′ exonuclease (proofreading) activity. The enzymes exhibit an error rate of approximately 1 error per 2.2 X 105 nucleotides incorporated. In hot start formulation, the KAPA Taq associates with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. PCR products generated with KAPA Taq are A-tailed and are suitable for cloning into TA cloning vectors.
Features and Benefits
High performance:
Quick Notes:
- Improved sensitivity, specificity, and yields
- Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product
Quick Notes:
- KAPA Taq ReadyMix can replace any commercial Taq DNA polymerase in an existing protocol. The annealing temperature may need to be optimized to account for differences in formulation.
- The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.
- The 2X KAPA Taq ReadyMix with dye includes two inert tracking dyes, which allow loading of PCR products directly onto agarose gels for analysis.
- KAPA Taq ReadyMixes contain 1.5 mM MgCl2 and 0.2 mM of each dNTP (at 1X).
General description
KAPA Taq ReadyMix™ (2X) is a ready-to-use cocktail containing all components for polymerase chain reaction (PCR), except primers and template. KAPA Taq ReadyMix is available with and without dye. The 2X ReadyMix with dye contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution. KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for longterm storage
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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