We do not provide a recommended protocol for 384-well conversion. However, the Custom Assay Team (CADI) offers this as a service.
48-680MAG
MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay
Synonym(s):
ERK Cell Signaling Assay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay
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96 WELLS
$3,180.00
$3,180.00
Product Availability: You will receive an order acknowledgement once the order has been entered in our system.
About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000
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species reactivity
human
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
storage temp.
2-8°C
Quality Level
detection method
fluorometric (Luminex® xMAP® technology)
1 of 4
This Item | 48-681MAG | 48-690MAG | 48-618MAG |
|---|---|---|---|
| species reactivity human | species reactivity human, mouse, rat | species reactivity human | species reactivity human, mouse, rat |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex® xMAP®) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
Analyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (Thr180/Tyr182), p70S6K (Thr412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473).
Research Category
Cell Signaling
Cell Signaling
Research Sub Category
Oncology
Oncology
Used to detect/quantify:CREB (pS133)|ERK (pT185/pY187)|NFκB (pS536)|JNK (pT183/pY185)|p38 (Thr180/Tyr182)|p70S6K (Thr412)|STAT3 (pS727)|STAT5A/B (pY694/699)|Akt (pS473)
Biochem/physiol Actions
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
General description
The Milliplex® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70S6K (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.
Preparation Note
Recommended storage for kit components is 2 - 8°C
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Christopher P Stanley et al.
Pharmacological research, 113(Pt A), 356-363 (2016-10-21)
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International journal of molecular sciences, 20(3) (2019-01-27)
The endothelial-to-mesenchymal transition (EndMT) is a biological process where endothelial cells (ECs) acquire a fibroblastic phenotype after concomitant loss of the apical-basal polarity and intercellular junction proteins. This process is critical to embryonic development and is involved in diseases such
Lee A Denson et al.
Inflammatory bowel diseases, 25(3), 547-560 (2018-08-21)
Granulocyte-macrophage colony-stimulating factor auto-antibodies (GMAbs) suppress neutrophil-extrinsic GM-CSF signaling and increase risk for stricturing behavior in Crohn's disease (CD). We aimed to define clinical, genomic, and functional associations with neutrophil-intrinsic GM-CSF signaling. Missense mutations in CSF2RA, CSF2RB, JAK2, STAT5A, and
Anna Coulibaly et al.
Scientific reports, 11(1), 7023-7023 (2021-03-31)
Natural killer (NK) cells mediate innate host defense against microbial infection and cancer. Hypoxia and low glucose are characteristic for these tissue lesions but do not affect early interferon (IFN) γ and CC chemokine release by interleukin 15 (IL-15) primed
Paul S Hafen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 125(5), 1447-1455 (2018-07-20)
The heat stress response is associated with several beneficial adaptations that promote cell health and survival. Specifically, in vitro and animal investigations suggest that repeated exposures to a mild heat stress (~40°C) elicit positive mitochondrial adaptations in skeletal muscle comparable
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Is there a protocol available for running the 96-well Milliplex kits in a 384-well format?
1 answer-
Helpful?
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Can you explain what the Amplification Buffer is in Cell Signaling Panels?
1 answer-
The use of the Amplification Buffer is proprietary. Its primary purpose is to enhance the signal of phosphorylated proteins.
Helpful?
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Are the Milliplex Cell Signaling panels qualitative? If so, what settings are used in xPonent for analysis? Can the data be normalized in Belysa?
1 answer-
The Cell Signaling kits are qualitative, and the xPonent Analysis method is not applicable. Belysa does not offer a qualitative option.
Helpful?
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