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Merck

1.40011

modified Tryptic Soy Broth- Dehydrated Culture Media

GranuCult® prime, according to USDA-FSIS, granular, suitable for microbiology, pathogen testing

Synonym(s):

mTSB, soy broth, CASO Broth, Casein-peptone Soymeal-peptone Broth, mTSB (modified Tryptic Soy Broth)

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213

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Product Name

modified Tryptic Soy Broth- Dehydrated Culture Media, GranuCult® prime, according to USDA-FSIS, granular, suitable for microbiology, pathogen testing

agency

according to USDA-FSIS

form

granular

manufacturer/tradename

GranuCult® prime

application(s)

food and beverages
microbiology
pathogen testing

suitability

Escherichia coli O157:H7
Escherichia coli (non-O157 Shiga Toxin-Producing (STEC))
Salmonella spp.

Quality Level

200

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1 of 4

This Item
1.092051.463161.46432
modified Tryptic Soy Broth- Dehydrated Culture Media GranuCult® prime, according to USDA-FSIS, granular, suitable for microbiology, pathogen testing

Millipore

1.40011

modified Tryptic Soy Broth- Dehydrated Culture Media

modified Tryptic Soy Broth- Dehydrated Culture Media GranuCult® prime, Novobiocin 20 mg/L, ISO 16654, granular, selective for Escherichia coli

Millipore

1.09205

modified Tryptic Soy Broth- Dehydrated Culture Media

Tryptic Soy Broth- Ready-to-use Bags, Tubes & Ampoules closure type, with MPC connector and triple-bagged, bag of 1 × 10 L

Millipore

1.46316

Tryptic Soy Broth- Ready-to-use Bags, Tubes & Ampoules

Tryptic Soy Broth- Ready-to-use Bags, Tubes & Ampoules tube capacity 17 mL, tube filling volume 9 mL, closure type, blue screw cap with 2 loci, box of 100 tubes

Millipore

1.46432

Tryptic Soy Broth- Ready-to-use Bags, Tubes & Ampoules

application(s)

food and beverages, microbiology
pathogen testing

application(s)

food and beverages
microbiology
pathogen testing

application(s)

aseptic process simulation
pharmaceutical

application(s)

cosmetics
food and beverages
pharmaceutical
sterility testing

form

granular

form

granular

form

liquid (ready-to-use)

form

liquid

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

GranuCult® prime

manufacturer/tradename

-

manufacturer/tradename

-

suitability

Escherichia coli O157:H7, Salmonella spp., Escherichia coli (non-O157 Shiga Toxin-Producing (STEC))

suitability

Escherichia coli (O157:H7 ), selective for Escherichia coli (EHEC), urea-degrading bacteria

suitability

nonselective for

suitability

nonselective for

agency

according to USDA-FSIS

agency

FDA-BAM, ISO 16654

agency

EP, USP

agency

EP 2.6.1, JP 4.06, USP 71

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

Application

modified Tryptic Soy Broth has emerged as the medium of choice for the enrichment of E. coli and Salmonella species in red meats. This medium is not intended for use in the diagnosis of a disease or other conditions in humans.

Features and Benefits

mTSB media renders the isolation simpler, less costly, and substantially suppresses the growth of other gram-negative Enterobacteriaceae species by 90%.

General description

mTSB (modified Tryptic Soy Broth) without the addition of novobiocin is a selective enrichment media for the isolation and growth of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) and Salmonella from meat products, carcass, and environmental sponges. For the selective enrichment of Escherichia coli O157:H7 in outbreak-related samples, e.g. fermented sausage, cooked meat products, the addition of novobiocin is recommended. Pancreatic digest of casein, papaic digest of soybean meal, and casamino acids (casein acid hydrolysate) serve as carbon and nitrogen sources, vitamins, and minerals. Glucose also serves as a carbon energy source for rapid growth during the enrichment. Sodium chloride maintains the osmotic balance and dipotassium hydrogen phosphate acts as a buffering agent for maintaining the pH balance of the medium. The addition of Bile salts ensures selectivity of the media.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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