This product is quite stable when stored in tablet form at 2-8°C. While storing the tablets at 20°C is not expected to be detrimental, stability studies have not been conducted. Upon reconstitution, prepared solutions are stable at least 2 weeks at 2-8°C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/291/s8830dat-ms.pdf
S8830
Protease Inhibitor Cocktail
SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins
Synonym(s):
Protease Inhibitor Cocktail
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2 TABS
CHF 65.20
20 TABS
CHF 794.00
About This Item
Form:
tablet
Storage temp.:
2-8°C
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Product Name
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
biological source
bovine lung
Quality Level
form
tablet
storage temp.
2-8°C
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| form tablet | form tablet | form DMSO solution | form tablet |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level - |
| biological source bovine lung | biological source bovine lung, bovine milk | biological source - | biological source - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
General description
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection.
Application
The broad specificity of SIGMAFAST™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select® media.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic, and metalloproteases.
Features and Benefits
EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Physical form
Tablet that is water soluble
Preparation Note
One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Other Notes
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Related Content
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S8830-2TAB | 04061835518449 |
| S8830-20TAB | 04061835518432 |
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Can the tablets be stored at -20C? Any stability data around this?
1 answer-
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Can we use S8830-SIGMA FAST, EDTA-Free, tablet for plant extract also?
1 answer-
This product has not been tested for use with plants. For applications involving plants, product P9599, Protease Inhibitor Cocktail, is recommended for plant cell and tissue extracts.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 answer-
The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
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What is the difference between Protease Inhibitors P8849 and S8830?
1 answer-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 answer-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 answer-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
1 answer-
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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