Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

View All Documentation

I1284

Sigma-Aldrich

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

Synonym(s):

IPTG solution

Sign Into View Organizational & Contract Pricing

Select a Size

5 ML
$90.90

$90.90

In StockDetails

All Photos(3)

Select a Size

Change View
5 ML
$90.90

About This Item

Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
367-93-1
Molecular Weight:
238.30
Beilstein/REAXYS Number:
4631
MDL number:
MFCD00063273
UNSPSC Code:
12352200
PubChem Substance ID:
24895928
NACRES:
NA.52

$90.90

In StockDetails

grade

for molecular biology

Quality Level

200

description

non-ionic

form

liquid

mol wt

238.3

shipped in

dry ice

storage temp.

−20°C

SMILES string

CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

Related Categories

General description

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Application

Isopropyl β-D-thiogalactopyranoside solution is:
  • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli[1] and bioactive peptides[2]
  • used in its half-life and decay studies[3]
  • suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Biochem/physiol Actions

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.[4]

Principle

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000398760
0000394486
0000398760
0000394486
0000236532

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots
Sukhanova A, et al.
Protocol Exchange, 10 (2012)
Half-life measurements of chemical inducers for recombinant gene expression
Pasotti L, et al.
Journal of Biological Engineering, 8(1), 5-5 (2014)
Random sequences are an abundant source of bioactive RNAs or peptides
Neme R, et al.
Nature ecology & evolution, 1(6), 0127-0127 (2017)
L H Hansen et al.
Current microbiology, 36(6), 341-347 (1998-06-03)
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with
Iltaf Ahmed et al.
Human molecular genetics, 24(11), 3172-3180 (2015-02-24)
There are two known mRNA degradation pathways, 3' to 5' and 5' to 3'. We identified likely pathogenic variants in two genes involved in these two pathways in individuals with intellectual disability. In a large family with multiple branches, we
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service