P5420
Pullulanase from Klebsiella pneumoniae
ammonium sulfate suspension, ≥5 units/mg protein (biuret)
Synonym(s):
Amylopectin 6-gluconohydrolase, Limit dextrinase
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About This Item
form
ammonium sulfate suspension
specific activity
≥5 units/mg protein (biuret)
storage temp.
2-8°C
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Application
Pullulanase is a glycolytic enzyme from Klebsiella pneumoniae that is used to hydrolyze pullulan, an α-1,6-linked homopolymer of maltotriose[1]. Product P5420 has been used to degrade carbohydrate from white beans Phaseolus vulgaris L. in healthy humans[2] and to study pullulan biosynthesis[1].
The enzyme from Sigma has been used to estimate the pullulan content of the ethanol precipitates obtained from fermented agro-industrial wastes.[3] It has also been used to the evaluate the branching of the maltodextrin fractions obtained from potato starch-derived maltodextrins (Paselli MD6) using ethanol precipitation methods.[4]
Biochem/physiol Actions
Pullulanase hydrolyzes α-1,6 linkages of starch.[1] Apart from pullulan, the enzyme also hydrolyzes α-1,6 linkages of amylopectin and glycogen. Maltose is the smallest sugar that it can release from an α-(1,6)-linkages.
Unit Definition
One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.2
Preparation Note
Highly purified by a modification of ion exchange chromatography.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Fractionation of maltodextrins by ethanol
Defloor I, et al.
Journal of Chromatography A, 803 (1-2), 103-109 (1998)
M A Finkelman et al.
Applied and environmental microbiology, 43(2), 483-485 (1982-02-01)
A simple radiometric microassay for extracellular polysaccharide elaboration by yeast-like cells of Aureobasidium pullulans was developed, based upon a procedure originally described by Catley (FEBS Lett. 20:174-176, 1972). Incorporation of [C]glucose into pullulan was linear with respect to time and
Pullulan content of the ethanol precipitate from fermented agro-industrial wastes.
Israilides CJ, et al.
Applied Microbiology and Biotechnology, 49(5), 613-617 (1998)
L Noah et al.
The Journal of nutrition, 128(6), 977-985 (1998-06-18)
Resistant starch (RS) is thought to be present in large amounts in legume seeds; however, it has never been quantified in healthy humans. RS from cooked (atmospheric pressure) white beans was quantified in humans and pigs, and characterized to explain
Ghina Ali et al.
Carbohydrate polymers, 93(1), 109-115 (2013-03-08)
Pullulanase is an enzyme that hydrolyses the α-1,6 linkages of pullulan (Pull) to produce maltotriose units. We studied the capacity of pullulanase to cleave its modified substrate: carboxymethylpullulan (CMPull), synthesized with two different degrees of substitution (DS=0.16 and 0.8). Size
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