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About This Item
UNSPSC Code:
12352207
MDL number:
Biological source:
bovine fetus
Origin:
Australia origin
Sterility:
γ-irradiated, sterile-filtered
Shipped in:
dry ice
biological source
bovine fetus
Quality Segment
sterility
γ-irradiated, sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤0.18 mg/mL
origin
Australia origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.
Biochem/physiol Actions
Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.
Analysis Note
- Endotoxin and hemoglobin tested
Other Notes
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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