MABE913

Anti-Ikaros Antibody, clone Ik14

clone Ik14, from mouse

• Synonym(s): DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: Ik14, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable (ChIP-seq); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001207694
• UniProt accession no.: Q13422
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IKZF1(10320)

Description

General description

Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.

Immunogen

Recombinant protein corresponding to Human Ikaros.

Application

Anti-Ikaros, clone Ik14 is an antibody against Ikaros for use in WB, ChIP, ChIP-seq.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Chromatin Biology

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ikaros in 10 µg of HL-60 and human lymph node lysate.
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones Ik14 and E9 (catalogue numbers MABE913 and MABE912 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).
ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).

Quality

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 0.5 µg/mL dilution of this antibody detected Ikaros in 10 µg of Jurkat cell lysate.

Target description

~60 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable