MABT217

Anti-Calreticulin Antibody, clone 16B11.1

clone 16B11.1, from mouse

• Synonym(s): Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 16B11.1, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_004334
• UniProt accession no.: P27797
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CALR(811)

Description

General description

Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters. Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER. Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression.

Immunogen

GST-tagged recombinant protein corresponding to human Calreticulin.

Application

Immunohistochemistry Analysis: A 1:300-1,000 dilution from a representative lot detected Calreticulin in human colorectal cancer and human tonsil tissues.

Flow Cyometry Analysis: A 1:500 dilution from a representative lot detected Calreticulin in non serum starved HeLa cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calreticulin on 10 µg of HeLa cell lysate.

Target description

~48 kDa observed

Linkage

Replaces: MABT145

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable