MABN238
Anti-Glucagon Antibody, clone 13D11.33
clone 13D11.33, from mouse
Synonym(s):
4030-01F, 4031-01F, AB932
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
13D11.33, monoclonal
species reactivity
human, rat
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
multiplexing: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GCG(2641)
General description
Glucagon is a hormone that is proteolytically cleaved into at least eight different functional peptides. It is produced by pancreatic cells and it has binding sites in the brain, intestines, and liver, among other tissues. Glucagon is secreted in response to hypoglycemia. Through the combined activation of Gsα- and Gq-coupled transmembrane receptors and their downstream signaling pathways, glucagon stimulates the release of glucose from the liver into the bloodstream. One important mechanism underlying this process is the activation of protein kinase A (PKA). PKA signals result in increased transcription of phosphoenolpyruvate carboxykinase, which encodes the rate-limiting enzyme in the production of glucose from noncarbohydrate substrates. PKA signals may also increase the breakdown of glycogen, and suppress glycolysis. Glucagon may also engage in calcium-dependent signaling via PLC. The importance of glucagon in regulating glucose homeostasis, in conjunction with insulin, is underscored by findings that glucagon and glucagon receptor signaling are disrupted in type II diabetes.
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human Glucagon.
Application
Detect Glucagon using this Anti-Glucagon Antibody, clone 13D11.33 validated for use in IHC(P), Immunofluorescence, ELISA, Multiplexing.
Immunohistochemistry Analysis: A 1:8,000 dilution from a representative lot detected Glucagon in normal rat pancreas tissue (For enhanced staining results, it is recommended to use a 10,000 dilution of this antibody.).
ELISA Analysis: A representative lot detected Glucagon in ELISA.
Luminex® Analysis: A representative lot detected Glucagon in Luminex Assay.
ELISA Analysis: A representative lot detected Glucagon in ELISA.
Luminex® Analysis: A representative lot detected Glucagon in Luminex Assay.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Quality
Evaluated by Immunofluorescence in normal rat pancreas tissue.
Immunofluorescence Analysis: A 1:8,000 dilution of this antibody detected Glucagon in normal rat pancreas tissue.
Immunofluorescence Analysis: A 1:8,000 dilution of this antibody detected Glucagon in normal rat pancreas tissue.
Target description
21 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Normal rat pancreas tissue
Normal rat pancreas tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
Luminex is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Darian T Carroll et al.
Frontiers in endocrinology, 15, 1417437-1417437 (2024-08-08)
Using a non-human primate (NHP) model of maternal Western-style diet (mWSD) feeding during pregnancy and lactation, we previously reported altered offspring beta:alpha cell ratio in vivo and insulin hyper-secretion ex vivo. Mitochondria are known to maintain beta-cell function by producing
Jing Liu et al.
Developmental cell, 48(1), 49-63 (2019-01-09)
In the developing pancreas, transient Neurog3-expressing progenitors give rise to four major islet cell types: α, β, δ, and γ; when and how the Neurog3+ cells choose cell fate is unknown. Using single-cell RNA-seq, trajectory analysis, and combinatorial lineage tracing
Chandan Sona et al.
JCI insight, 7(6) (2022-02-09)
BACKGROUNDPathophysiology of type 1 diabetes (T1D) is illustrated by pancreatic islet infiltration of inflammatory lymphocytes, including CD8+ T cells; however, the molecular factors mediating their recruitment remain unknown. We hypothesized that single-cell RNA-sequencing (scRNA-Seq) analysis of immune cell populations isolated
Peter A Kropp et al.
American journal of physiology. Endocrinology and metabolism, 314(4), E308-E321 (2018-01-20)
The transcription factors pancreatic and duodenal homeobox 1 (Pdx1) and onecut1 (Oc1) are coexpressed in multipotent pancreatic progenitors (MPCs), but their expression patterns diverge in hormone-expressing cells, with Oc1 expression being extinguished in the endocrine lineage and Pdx1 being maintained
Karin J Bosma et al.
Molecular metabolism, 54, 101347-101347 (2021-10-10)
Type 2 diabetes is characterized by hyperglycemia and inflammation. Prostaglandin E2, which signals through four G protein-coupled receptors (EP1-4), is a mediator of inflammation and is upregulated in diabetes. We have shown previously that EP3 receptor blockade promotes β-cell proliferation
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