MAB2160
Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3
ascites fluid, clone 1C3, Chemicon®
Synonym(s):
FMRP
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1C3, monoclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
suitability
not suitable for flow cytometry
not suitable for immunoprecipitation
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... FRAXA(2477) , FRAXA(108684022)
General description
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
Specificity
Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.
Immunogen
Fusion protein with a full length FMRP (human).
Application
Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.
Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
RNA Binding Protein (RBP)
Neurodegenerative Diseases
RNA Binding Protein (RBP)
Quality
Evaluated by Western Blot on Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
Target description
~71 kDa
Physical form
Ascites mouse monoclonal IgG1k fluid containing no preservatives
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity.
Bartley, CM; O'Keefe, RA; Bordey, A
Testing null
Gabi Schutzius et al.
Journal of neurodevelopmental disorders, 5(1), 8-8 (2013-04-04)
Hypermethylation of the fragile X mental retardation 1 gene FMR1 results in decreased expression of FMR1 protein FMRP, which is the underlying cause of Fragile X syndrome - an incurable neurological disorder characterized by mental retardation, anxiety, epileptic episodes and
The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, ME; Bisson, N; Davidovic, L; Mazroui, R; Labelle, Y; Moss, T; Khandjian, EW
Molecular Biology of the Cell null
Julie B Zang et al.
PLoS genetics, 5(12), e1000758-e1000758 (2009-12-17)
The mental retardation, autistic features, and behavioral abnormalities characteristic of the Fragile X mental retardation syndrome result from the loss of function of the RNA-binding protein FMRP. The disease is usually caused by a triplet repeat expansion in the 5'UTR
Kathryn B Spencer et al.
Alcoholism, clinical and experimental research, 40(6), 1251-1261 (2016-05-06)
Exposure to chronic ethanol (EtOH) results in changes in the expression of proteins that regulate neuronal excitability. This study examined whether chronic EtOH alters the hippocampal expression and function of fragile X mental retardation protein (FMRP) and the role of
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