HTS191RTA
Ready-to-Assay OXGR1 α-Ketoglutarate Receptor Frozen Cells
Human
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
Ready-to-Assay OXGR1 α-Ketoglutarate Receptor Frozen Cells,
biological source
human
Quality Level
manufacturer/tradename
Ready-to-Assay
technique(s)
calcium flux assay: suitable
NCBI accession no.
detection method
fluorometric
General description
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
OXGR1 (also known as GPR99 or GPR80) was originally identified as an orphan with a sequence most closely related to P2Y receptors. Studies to deorphanize OXGR1 revealed that α-ketoglutarate activates OXGR1 through Gq to increase intracellular calcium (He et al., 2004; Qi et al., 2004; Abbracchio et al., 2005). The EC50 of OXGR1 for α-ketoglutarate is in the μM range, similar to the concentration of α-ketoglutarate in the circulation, indicating that the interaction is physiologically significant. Millipore′s cloned human OXGR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OXGR1-expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OXGR1.
OXGR1 (also known as GPR99 or GPR80) was originally identified as an orphan with a sequence most closely related to P2Y receptors. Studies to deorphanize OXGR1 revealed that α-ketoglutarate activates OXGR1 through Gq to increase intracellular calcium (He et al., 2004; Qi et al., 2004; Abbracchio et al., 2005). The EC50 of OXGR1 for α-ketoglutarate is in the μM range, similar to the concentration of α-ketoglutarate in the circulation, indicating that the interaction is physiologically significant. Millipore′s cloned human OXGR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OXGR1-expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OXGR1.
Cell Line Description
- GPCR Cell Lines
- Host cells: Chem-1
Application
Human OXGR1, GPR99, GPR80 GPCR frozen cells for Calcium Flux FLIPR Assays.
Biochem/physiol Actions
- GPCR Class: A
- Protein Target: OXGR, GPR99, GPR80
- Target Sub-Family: alpha-Ketoglutarate
Components
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Disclaimer
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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