06-1082-I

Anti-IL-27A Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, Interleukin-30, p28, IL-27A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_663634
• UniProt accession no.: Q8NEV9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IL27(246778)

Description

General description

Interleukin 27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits: Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-27A). IL-27 has pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. IL-27 is secreted by antigen-presenting cells and plays an important function in regulating the activity of B and T lymphocytes. IL-27 suppresses the expression of GATA3, the inhibitor of TH1 cell development. In addition to suppressing cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its anti-tumor activity as well as its anti-angiogenic activity with activation of production of anti-angiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increases the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Immunogen

KLH-conjugated linear peptide corresponding to human IL-27A.

Application

Anti-IL-27A Antibody is an antibody against IL-27A for use in Western Blotting, Immunohistochemistry and Flow Cytometry.

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected IL-27A in human placenta and human tonsil tissue.
Flow Cytometry Analysis: 0.5 µg of this antibody from a representative lot detected IL-27A in 1X10E6 staph enterotoxin B treated human peripheral blood mononuclear cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-27A in 10 µg of human placenta tissue lysate.

Target description

~27 kDa observed. Uncharacterized band(s) may appear in some lysates.

Linkage

Replaces: 06-1082

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable