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05-764

Sigma-Aldrich

Anti-MLL/HRX Antibody, NT., clone N4.4

clone N4.4, Upstate®, from mouse

Synonym(s):

Anti-ALL-1, Anti-ALL1, Anti-CXXC7, Anti-HRX, Anti-HTRX, Anti-HTRX1, Anti-MLL, Anti-MLL1, Anti-MLL1A, Anti-TRX1, Anti-WDSTS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

N4.4, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005933

UniProt accession no.

Q03164

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KMT2A(4297)

General description

The acute lymphoblastic leukaemia (ALL)-1 (also known as MLL, HRX, HTRX, and TRX1) gene on human chromosome 11q23 is the site of many locally clustered chromosomal alterations associated with several types of acute leukaemias, including deletions, partial duplications and translocations. Structurally variant proteins derived from the altered gene presumably cause the malignant transformation of early haemopoietic progenitor cells.

Specificity

Recognizes N-terminal proteolytic fragment of MLL (MLLN).

Immunogen

MBP fusion protein corresponding to residues 161-356 of human mixed lineage leukemia, MLL

Application

Detect MLL/HRX with Anti-MLL/HRX Antibody, N-term., clone N4.4 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

Quality

routinely evaluated by immunoblot on nuclear extract from K562 cells

Target description

300 kDa

Physical form

Format: Purified
Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anmaar M Abdul-Nabi et al.
PloS one, 5(8), e12464-e12464 (2010-09-02)
Different fusion oncogenes in acute myeloid leukemia (AML) have distinct clinical and laboratory features suggesting different modes of malignant transformation. Here we compare the in vitro effects of representatives of 4 major groups of AML fusion oncogenes on primary human
Chi Wai So et al.
Molecular and cellular biology, 22(18), 6542-6552 (2002-08-23)
MLL-AFX is a fusion gene created by t(X;11) chromosomal translocations in a subset of acute leukemias of either myeloid or lymphoid derivation. It codes for a chimeric protein consisting of MLL fused to AFX, a forkhead transcription factor that normally
Clara Bueno et al.
Cell research, 22(6), 986-1002 (2012-01-04)
The MLL-AF4 fusion gene is a hallmark genomic aberration in high-risk acute lymphoblastic leukemia in infants. Although it is well established that MLL-AF4 arises prenatally during human development, its effects on hematopoietic development in utero remain unexplored. We have created
Inducible MLL-AF9 Expression Drives an AML Program during Human Pluripotent Stem Cell-Derived Hematopoietic Differentiation.
Heuts, et al.
Cells, 12 (2023)
Matthew J Smith et al.
Nature communications, 8(1), 1099-1099 (2017-10-25)
Elucidation of activation mechanisms governing protein fusions is essential for therapeutic development. MLL undergoes rearrangement with numerous partners, including a recurrent translocation fusing the epigenetic regulator to a cytoplasmic RAS effector, AF6/afadin. We show here that AF6 employs a non-canonical
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