This product may be prepared in 50 mM TES buffer, pH 7.0, with 1 mM MgCl2, at 1 mg/mL. It is also soluble in water or TE buffer. Please see the link below to review the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/382/514/m9901pis-ms.pdf
M9901
Mutanolysin from Streptomyces globisporus ATCC 21553
lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Synonym(s):
Mutanolysin
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| Size/SKU | Availability | Price |
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1000 units | Available to ship TODAYfromMILWAUKEE | $80.10 |
5000 units | Estimated to ship onSeptember 15, 2026fromMILWAUKEE | $171.00 |
10000 units | Estimated to ship onSeptember 15, 2026fromMILWAUKEE | $245.00 |
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About This Item
Specific activity:
≥4000 units/mg protein (biuret)
Biological source:
bacterial (Streptomyces globisporus )
biological source
bacterial (
Quality Segment
form
lyophilized powder
specific activity
≥4000 units/mg protein (biuret)
mol wt
23 kDa
input
bacteria and yeast sample(s)
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control. PMID 15828186
Application
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. [1] It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Biochem/physiol Actions
Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.
Physical form
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Other Notes
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
Ficoll is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| description lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified | description Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret) | description 0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret) | description lyophilized powder, ≥200 units/mg protein |
| biological source bacterial ( | biological source bacterial ( | biological source - | biological source - |
| specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥4000 units/mg protein (biuret) | specific activity ≥200 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability - | suitability - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Hi, Could you advise the best way to resuspend mutanolysin M9901. Will it be soluble in water?
1 answer-
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