The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf
C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Synonym(s):
cryopreservation medium
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 mL | Estimated to ship TODAYfromSAINT LOUIS | $127.00 |
About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
$127.00
Estimated to ship TODAYDetails
sterility
sterile-filtered
Quality Segment
form
liquid
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable, cryopreservation: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Overview of good cell banking practices for cell line cryopreservation purposes.
Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.
Related Content
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
Kai Pollard et al.
Scientific data, 7(1), 184-184 (2020-06-21)
Nerve sheath tumors occur as a heterogeneous group of neoplasms in patients with neurofibromatosis type 1 (NF1). The malignant form represents the most common cause of death in people with NF1, and even when benign, these tumors can result in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C6295-50ML | 04061833511114 |
-
Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.
1 answer-
Helpful?
-