Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

APF

Sigma-Aldrich

Alkaline Phosphatase Detection Kit, Fluorescence

Sign Into View Organizational & Contract Pricing

Select a Size

1 KIT
$938.00

$938.00

Check Cart for Availability
All Photos(1)

Select a Size

Change View
1 KIT
$938.00

About This Item

EC Number:
245-325-0
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.53

$938.00

Check Cart for Availability

grade

Molecular Biology

Quality Level

200

usage

1 kit sufficient for 300 reactions (200 μl reactions)

technique(s)

microbe id | specific enzyme detection: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ALPL(249)

General description

The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.

Application

Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity.[1]
Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts.

Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Biochem/physiol Actions

Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways.[2]

Features and Benefits

  • Up to 100x more sensitive than colorimetric assays
  • SEAP can be measured without sample destrutcion
  • Rapid and reproducible results
  • Safe and easy-to-use

Components

The Alkaline Phosphatase Kit contains:
50mL Fluorescent Assay Buffer ( B6558)
6 mL Dilution Buffer (B6433)
50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361)
2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168)

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–3 of 3 Questions  

  1. Is Product APF, Alkaline Phosphatase Detection Kit, used for fluorometric or colorimetric assay?

    1 answer

    1. The assay used is fluorometric, which is more sensitive than the colorimetric assay.

      Helpful?

  2. Is Product APF, Alkaline Phosphatase Detection Kit, Fluorescence, used to measure the alkaline phosphatase (AP) activity of cell lysates or the AP activity secreted by cells?

    1 answer

    1. The technical bulletin provides procedures for both. The kit was originally designed for secreted alkaline phosphatase (SEAP), because the human placental SEAP standard that comes with the kit is a secreted enzyme. Its activity in the cell sample can be measured nondestructively and repeatedly over time using an aliquot of the culture medium. It saves time required for cell extract preparations.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service