MABC953
Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3
clone 7F8E3, from rat
Synonym(s):
Prohibitin-2, B-cell receptor-associated protein BAP37, D-prohibitin, Repressor of estrogen receptor activity, Prohibitin-2/PHB2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7F8E3, monoclonal
species reactivity
rat, monkey, human, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PHB2(11331)
General description
Prohibitin-2 (UniProt Q99623; also known as B-cell associated protein, B-cell receptor-associated protein BAP37, BCAP37, D-prohibitin, PHB2, Repressor of estrogen receptor activity) is encoded by the PHB2 (also known as BAP, REA) gene (Gene ID 11331) in human. Prohibitins are assembled into a ring-like PHB complex with 16-20 alternating Phb1 and Phb2 subunits in the inner mitochondrial membrane, where they function as chaperone for respiration chain proteins or as important structural scaffold for optimal mitochondrial morphology and function. Prohibitins are also reported to modulate transcriptions by interacting with various transcription factors, including nuclear receptors. Upregulated expression of prohibitins, septin 9, neurabin 1, and other cytoskeletal and functional proteins is found at the glutathione S-transferase placental-form-positive (GST-P+) hepatocellular carcinoma (HCC) foci during initiation and/or promotion stages of rat hepatocarcinogenesis, implicating an involvement of prohibitins in the neoplastic transformation of rat liver preneoplastic lesions.
Specificity
Expected to react with both spliced isoforms.
Immunogen
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Prohibitin-2/PHB2.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Prohibitin-2/PHB2 Antibody, clone 7F8E3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of Prohibitin-2/PHB2.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Prohibitin-2/PHB2 in 10 µg of COS-7 cell lysate.
Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue.
Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72).
Immunocytochemistry Analysis: 4 µg/mL from a representative lot detected Prohibitin-2/PHB2 in A431 and HUVEC cells.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Prohibitin-2/PHB2 in human breast and mouse liver tissue.
Immunohistochemistry Analysis: A representative lot detected upregulated Prohibitin-2 immunoreactivity at the glutathione S-transferase placental-form–positive (GST-P+) hepatocellular carcinoma (HCC) foci in frozen liver sections from rats subjected to hepatocarcinogenesis induction by N-nitrosodiethylamine (DEN) and Phenobarbital (PB) treatment (Kakehashi, A., et al. (2011). Toxicol Sci. 119(1):61-72).
Quality
Evaluated by Western Blotting in rat C6 glioma cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Prohibitin-2/PHB2 in 10 µg of rat C6 glioma cell lysate.
Target description
~34 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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