Branching frequency/degree of branching and hydrodynamic properties are not published. The end-group ratio is primary : secondary : tertiary = 1:1.2:0.76. As per the literature, branched polyethyleneimine is commonly described as about 25% primary, 50% secondary, and 25% tertiary, and is typically more compact in solution with limited viscosity changes across pH and ionic strength. Please see the link below to review publications that may be of interest.
Kobayashi et al., Polymer Journal (1990), “Viscosity Behaviors and Gel Properties of Linear and Branched Polyethylenimines: Effects of Micro-Structures”
https://www.nature.com/articles/pj199060.pdf
408727
Polyethylenimine, branched
average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched
Synonym(s):
aziridine, homopolymer, PEI
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Available to ship TODAYfromMILWAUKEE | $103.00 |
250 mL | Available to ship TODAYfromMILWAUKEE | $183.00 |
1 L | Available to ship TODAYfromMILWAUKEE | $494.00 |
About This Item
Linear Formula:
H(NHCH2CH2)nNH2
CAS Number:
NACRES:
NA.23
PubChem Substance ID:
UNSPSC Code:
12162002
MDL number:
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Quality Segment
form
viscous liquid
mol wt
average Mn ~10,000 by GPC, average Mw ~25,000 by LS
impurities
≤1% water
refractive index
n20/D 1.5290
viscosity
13,000-18,000(50 °C)
bp
250 °C (lit.)
density
1.030 g/mL at 25 °C
SMILES string
C1CN1
InChI
1S/C2H5N/c1-2-3-1/h3H,1-2H2
InChI key
NOWKCMXCCJGMRR-UHFFFAOYSA-N
General description
Polyethylenimine (PEI) is a hydrophilic cationic polymer widely used as a nonviral nucleotide delivery reagent. Branched PEI can be synthesized by cationic ring-opening polymerization of aziridine. PEI-based particles can also be used as adjuvants for vaccines. Owing to its excellent physicochemical properties, it is applied in many fields like the separation and purification of proteins, carbon dioxide absorption, drug carriers, effluent treatment, and biological labels.
Application
Polyethyleneimine can be used as a non-viral synthetic polymer vector for in vivo delivery of therapeutic nucleic acids. The interaction between negatively charged nucleic acids and positively charged polymer backbone results in the formation of nano-sized complexes. This neutralized complex protects the enclosed nucleic acid from enzymes and maintains its stability till the cellular uptake takes place. For example, human serum albumin conjugated PEI shows good pDNA transfection and low toxicity.
PEI can be used to functionalize single-walled nanotubes (SWNTs) to improve their solubility and biocompatibility while maintaining the structural integrity of the original SWNT. Covalently functionalized SWNTs find application in CO2 absorption and gene delivery.
Branched PEI can also be used to modify the surface properties of adsorbents. PEI-modified hydrous zirconium oxide/PAN nanofibers are used for the defluorination of groundwater as they show high fluoride adsorption capacity and a wide working pH range.
PEI can be used to functionalize single-walled nanotubes (SWNTs) to improve their solubility and biocompatibility while maintaining the structural integrity of the original SWNT. Covalently functionalized SWNTs find application in CO2 absorption and gene delivery.
Branched PEI can also be used to modify the surface properties of adsorbents. PEI-modified hydrous zirconium oxide/PAN nanofibers are used for the defluorination of groundwater as they show high fluoride adsorption capacity and a wide working pH range.
Features and Benefits
Primary and secondary amine groups of PEI can efficiently bind to drugs, nucleic acids, and other functional moieties.
Branched PEI has better complexation andbuffering capacity.
Branched PEI has better complexation andbuffering capacity.
Physical form
Branched polymer
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This Item | |||
|---|---|---|---|
| viscosity 13,000-18,000(50 °C) | viscosity - | viscosity - | viscosity - |
| refractive index n20/D 1.5290 | refractive index - | refractive index - | refractive index - |
| form viscous liquid | form solid | form liquid | form solid |
| density 1.030 g/mL at 25 °C | density - | density - | density - |
| mol wt average Mn ~10,000 by GPC, average Mw ~25,000 by LS | mol wt average Mn 10,000 | mol wt average Mn 2100 | mol wt average Mn 5,000 |
| bp 250 °C (lit.) | bp - | bp - | bp - |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Is there any additional information regarding branching frequency or ratio of primar, secondary and tertiary amines? Data related to branching structure and hydrodynamic behavior/compactness?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Do you have any GPC (Gel permeation chromatography) data for this product n°408727 that you can share?
1 answer-
This product is not tested by Gel Permeation Chromatography. The molecular weight distribution of this material has not been determined. However, historical information indicates the mole fraction of primary, secondary, and tertiary amines is 1:1.20:0.76. The material dispersity (Mw / Mn) is 25,000 / 10,000 = 2.5.
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Is Branched PEI soluble in Diethylene glycol and other glycols? Do these solvents affect the amine functions/charges?
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This compound is readily soluble in hot water and some organic solvents, such as methanol, ethanol, or chloroform. However, the solubility in diethylene glycol or other glycols has not been determined.
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Can this product be used for stable transfection?
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This product is not transfection grade and has not been tested as suitable for this type of application. It is the responsibility of the end user to determine suitability for this type of application. A search of the scientific literature does reveal that a few laboratories have reported the use of this product for transfection. Those article citations can be found below.
Hoenigsperger, H., Koepke, L., Acharya, D., Hunszinger, V., Freisem, D., Grenzner, A., … Sparrer, K. M. J. (2023). CSNK2 suppresses autophagy by activating FLN-NHL-containing TRIM proteins. Autophagy, 1–21. https://doi.org/10.1080/15548627.2023.2281128
Kritika Saxena, Nicola Domenico Roverato, Melody Reithmann, Mei Min Mah, Richard Schregle, Gunter Schmidtke, Ivan Silbern, Henning Urlaub, Annette Aichem. (2023) Phosphorylated FAT10 interferes with IFN-I signaling Life Science Alliance DOI: 10.26508/lsa.202101282
Kirman, D.C., Renganathan, B., Chui, W.K. et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13, 172 (2022). https://doi.org/10.1038/s41419-022-04618-x
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What recommended storage conditions (temperature) of PEI? Is refrigeration or freezing suitable? And how shelf-life at storage conditions? Impact of dilution on PEI's shelf-life ?
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Product no. 408727 Polyethylenimine, branched is stored at ambient temperature and does not have an assigned shelf-life.
We offered a 2 mg/ml Branched polyethylenimine solution (Product no. 913375) that was stable for 2 years at 2 - 8 degrees C.
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Can you please provide me with the number of primary amines per molecule for the product with the code 408727?
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Approximately 6.4 mmol of primary amines per gram of dry substance. This calculation is derived from the equation 1/(1+1.1+0.7) multiplied by 18, resulting in 6.4.
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Is this polymer soluble in THF?
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The solubility of this product is not tested on a lot-to-lot basis. This compound is readily soluble in water and sparingly soluble in organic solvents such as DMSO and acetone. Other sources report that this material may also be sparingly soluble in THF. This information has not been validated.
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What is the N(Nitrogen)% for following PEI? Polyethylenimine, branched, average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched, 250ml
1 answer-
Unfortunately, the N content of Product 408727 is not determined.
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