HPLC Analysis of Proteins on Mono Q HR 5/5

column

Mono Q HR 5/5, 10 μm particles (54807)

mobile phase

[A]: 20 mM bis-Tris, pH 6.0; [B]: A + 1M sodium chlorde

flow rate

1.0 mL/min

detector

UV, 280 nm

injection

2.5 mL

sample

human placental lactogen fraction from size exclusion medium