Skip to Content
Merck

Maleimidyl magnetic nanoplatform for facile molecular MRI.

Nanotechnology (2014-06-25)
Dan Heo, Eugene Lee, Minhee Ku, Seungyeon Hwang, Bongjune Kim, Yeonji Park, Young Han Lee, Yong-Min Huh, Seungjoo Haam, Jae-Ho Cheong, Jaemoon Yang, Jin-Suck Suh
ABSTRACT

In this study, we developed the maleimidyl magnetic nanoplatform, which enables functional targeting of a biomarker-specific moiety for molecular imaging via MRI. The maleimide group of the maleimidyl magnetic nanoplatform is conjugated with a thiol group without additional crosslinkers and side products. A physicochemical analysis was conducted to verify the effectiveness of the maleimidyl magnetic nanoplatform, and the existence of the maleimidyl group was investigated using the platform. To prepare biomarker-specific MRI probes, a thiolated aptamer and peptide were immobilized onto the maleimidyl group of the maleimidyl magnetic nanoplatform. The fabricated MRI probes were applied to four cancer cell lines: HT1080, MCF7, MKN45, and HEK293T. To investigate the potential of the molecular MRI probe, the target-biomarker specificity was confirmed without serious cytotoxicity, and in vivo MRI analysis using a xenograft mouse model was demonstrated. We believe these results will be useful for fabricating molecular MRI probes for the diagnosis of cancer.

MATERIALS
Product Number
Brand
Product Description

USP
Valacyclovir Related Compound G, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Triethylamine, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
Dodecylamine, puriss., ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)
Sigma-Aldrich
Dodecylamine, ≥99%
Sigma-Aldrich
Dodecylamine, 98%
Sigma-Aldrich
Triethylamine, for amino acid analysis, ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
Triethylamine, ≥99.5%
Sigma-Aldrich
Triethylamine, ≥99%
Sigma-Aldrich
Triethylamine, ≥99.5%
Supelco
Triethylamine, analytical standard
Valaciclovir impurity G, European Pharmacopoeia (EP) Reference Standard
Supelco
Dichloromethane, Selectophore, ≥99.5%
Sigma-Aldrich
DCC, 1.0 M in methylene chloride
Sigma-Aldrich
Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Benzyl ether, 98%
Sigma-Aldrich
N-Maleoyl-β-alanine, 97%
Sigma-Aldrich
Benzyl ether, ≥98%, FCC, FG
Supelco
Dichloromethane, analytical standard
Sigma-Aldrich
Dibenzyl ether, purum, ≥98.0% (GC)
Sigma-Aldrich
DCC, puriss., ≥99.0% (GC)
Supelco
Dichloromethane, ≥99.9%
Sigma-Aldrich
DCC, 99%
Sigma-Aldrich
Manganese(II) acetylacetonate
Sigma-Aldrich
4-(Dimethylamino)pyridine, purum, ≥98.0% (NT)
Supelco
Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Tribenoside impurity D, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer