MAB4301
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480
clone MC-480, Chemicon®, from mouse
Synonym(s):
SSEA-1, CD15, Lewis X, 3-FAL
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
MC-480, monoclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... FUT4(2526)
General description
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Specificity
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Immunogen
F9 tetracarcinoma stem cells (X-irradiated).
Application
Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.
Immunohistochemistry:
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
A previous lot of this antibody was tested on IH.
Immunoprecipitation:
A previous lot of this antibody was tested on IP.
Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.
Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.
Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Neural Stem Cells
Pluripotent & Early Differentiation
Neural Stem Cells
Quality
Tested
Target description
220 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
P19 mouse embryonic carcinoma cells
P19 mouse embryonic carcinoma cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Efficient generation of iPS cells from skeletal muscle stem cells.
Tan, KY; Eminli, S; Hettmer, S; Hochedlinger, K; Wagers, AJ
Testing null
Hans M Larsson et al.
PloS one, 7(11), e49874-e49874 (2012-12-05)
While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary
Haiyang Zhang et al.
Urology, 79(3), 746-746 (2011-12-27)
To identify potential stem cells in urinary bladder by label-retaining cell (LRC) strategy and immunostaining for putative stem cell markers. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders
Yan-Min Feng et al.
Cell cycle (Georgetown, Tex.), 13(5), 782-791 (2014-01-09)
A critical process of early oogenesis is the entry of mitotic oogonia into meiosis, a cell cycle switch regulated by a complex gene regulatory network. Although Notch pathway is involved in numerous important aspects of oogenesis in invertebrate species, whether
Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis.
Muller, T; Eildermann, K; Dhir, R; Schlatt, S; Behr, R
Human Reproduction null
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