ABN1640

Anti-Prodynorphin/Leumorphin (dynorphin B29)

serum, from rabbit

• Synonym(s): Preproenkephalin-B, Proenkephalin-B, Beta-neoendorphin-dynorphin, Leumorphin, Dynorphin B-29
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• technique(s): western blot: suitable
• NCBI accession no.: NP_062247
• UniProt accession no.: P06300
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PDYN(5173) ; rat ... Pdyn(29190)

Description

General description

Proenkephalin-B (UniProt P06300; also known as Beta-neoendorphin-dynorphin, Preprodynorphin) is encoded by the Pdyn gene (Gene ID 29190) in rat species. Proenkephalin B is an opioid polypeptide hormone involved with chemical signal transduction and cell communication. Prodynorphin is a basic building-block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds critical in learning and memory. Prodynorphin is produced with a signal peptide (a.a. 1-21) and a propeptide (a.a. 22-163) sequence. Following the removal of the signal and propeptide sequences, the protein is further processed by proeolytic cleavages to form Alpha-neoendorphin (a.a. 166-175), Beta-neoendorphin (a.a. 166-174), Big dynorphin (a.a. 202-233; Big Dyn), Dynorphin A(1-17) (a.a. 202-218; Dyn-A17; Dynorphin A); Dynorphin A(1-13) (a.a. 202-214), Dynorphin A(1-8) (a.a. 202-209), Leu-enkephalin (a.a. 202-206 & 221-225), Leumorphin (Dynorphin B-29; a.a. 221-248), and Rimorphin (a.a. 221-233; Dynorphin B; Dyn-B; Dynorphin B(1-13)).

Specificity

This polyclonal antiserum recognizes preproenkephalin-B (a.a. 1-248 of rat sequence), proenkephalin-B (a.a. 22-248 of rat sequence), and leumorphin/dynorphin B-29 (a.a. 221-248 of rat sequence), but not other peptides derived from Proenkephalin-B.

Immunogen

Epitope: Leumorphin peptide.

Linear peptide corresponding to rat leumorphin (dynorphin B-29) C-terminal half sequence.

Application

Anti-Prodynorphin Antibody is an antibody against Leumorphin (dynorphin B29) for use in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected leumorphin (dynorphin B29) in 10 µg of human brain tissue lysate.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected prodynorphin in 10 µg of rat brain tissue lysate.

Target description

~36/3.5 kDa oberved. 28.08/28.06/28.39 kDa (Rat/Mouse/Human preproenkephalin-B), 25.85/25.76/26.28 kDa (Rat/Mouse/Human proenkephalin-B), 3.320/3.320/3.352 kDa (Rat/Mouse/Human leumorphin) calculated. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable