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05-829-AF555

Sigma-Aldrich

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate

clone DM1A, from mouse, ALEXA FLUOR™ 555

Synonym(s):

Tubulin alpha-1 chain, Alpha-tubulin, Alpha-1 tubulin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified antibody

antibody product type

primary antibodies

clone

DM1A, monoclonal

species reactivity

guinea pig, gerbil, mouse, bovine, avian, human, rat, porcine

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_001292201.1

UniProt accession no.

P02552

target post-translational modification

unmodified

General description

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

Specificity

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

Immunogen

Epitope: C-terminal 25 amino acids.
Purified chicken brain α-tubulin.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
This Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.

Target description

~50 kDa observed.

Physical form

Protein G purified.
Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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