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  • Infliximab relieves blood retinal barrier breakdown through the p38 MAPK pathway in a diabetic rat model.

Infliximab relieves blood retinal barrier breakdown through the p38 MAPK pathway in a diabetic rat model.

International journal of ophthalmology (2017-12-21)
Mao-Song Xie, Yong-Zheng Zheng, Li-Bin Huang, Guo-Xing Xu
ABSTRACT

To clarify the mechanism of infliximab treatment in diabetic macular edema (DME) and to provide a new alternative therapy for DME. Rats were randomly divided into the control group, the model group and the infliximab treatment group. A diabetic rat model was created. The concentration of TNF-α in the vitreous body was detected by ELISA. The expressions of B-Raf, p38, claudin-1 and occludin in the retina were detected by Western blot. The integrity of the blood retinal barrier (BRB) was measured using Evan's blue as a tracer. After three months and six months of the diabetes model, the vitreous TNF-α level in the model group was higher than that of the control group. It was also higher in treated group than that of the control group but was lower than that of the model group. The differences among the three groups were statistically significant (at 3mo, In a diabetic rat model, infliximab may relieve TNF-α induced BRB breakdown