Skip to Content
Merck
  • Bmp2 conditional knockout in osteoblasts and endothelial cells does not impair bone formation after injury or mechanical loading in adult mice.

Bmp2 conditional knockout in osteoblasts and endothelial cells does not impair bone formation after injury or mechanical loading in adult mice.

Bone (2015-09-08)
Sarah Howe McBride-Gagyi, Jennifer A McKenzie, Evan G Buettmann, Michael J Gardner, Matthew J Silva
ABSTRACT

Post-natal osteogenesis after mechanical trauma or stimulus occurs through either endochondral healing, intramembranous healing or lamellar bone formation. Bone morphogenetic protein 2 (BMP2) is up-regulated in each of these osteogenic processes and is expressed by a variety of cells including osteoblasts and vascular cells. It is known that genetic knockout of Bmp2 in all cells or in osteo-chondroprogenitor cells completely abrogates endochondral healing after full fracture. However, the importance of BMP2 from differentiated osteoblasts and endothelial cells is not known. Moreover, the importance of BMP2 in non-endochondral bone formation such as intramembranous healing or lamellar bone formation is not known. Using inducible and tissue-specific Cre-lox mediated targeting of Bmp2 in adult (10-24 week old) mice, we assessed the role of BMP2 expression globally, by osteoblasts, and by vascular endothelial cells in endochondral healing, intramembranous healing and lamellar bone formation. These three osteogenic processes were modeled using full femur fracture, ulnar stress fracture, and ulnar non-damaging cyclic loading, respectively. Our results confirmed the requirement of BMP2 for endochondral fracture healing, as mice in which Bmp2 was knocked out in all cells prior to fracture failed to form a callus. Targeted deletion of Bmp2 in osteoblasts (osterix-expressing) or vascular endothelial cells (vascular endothelial cadherin-expressing) did not impact fracture healing in any way. Regarding non-endochondral bone formation, we found that BMP2 is largely dispensable for intramembranous bone formation after stress fracture and also not required for lamellar bone formation induced by mechanical loading. Taken together our results indicate that osteoblasts and endothelial cells are not a critical source of BMP2 in endochondral fracture healing, and that non-endochondral bone formation in the adult mouse is not as critically dependent on BMP2.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Methyl methacrylate, contains ≤30 ppm MEHQ as inhibitor, 99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Methyl methacrylate, 99%, stabilized
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, contains ethanol as stabilizer, ACS reagent, ≥99.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, contains amylenes as stabilizer, ACS reagent, ≥99.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), Grade II, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, Grade I, ≥99% (GC), suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioXtra
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ACS reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00
Sigma-Aldrich
Tamoxifen, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
1 Estimated to ship on 08 March 2025
CHF 88.00