Skip to Content
Merck
  • Comparative cytotoxicity of artemisinin and cisplatin and their interactions with chlorogenic acids in MCF7 breast cancer cells.

Comparative cytotoxicity of artemisinin and cisplatin and their interactions with chlorogenic acids in MCF7 breast cancer cells.

ChemMedChem (2014-09-12)
John O Suberu, Isolda Romero-Canelón, Neil Sullivan, Alexei A Lapkin, Guy C Barker
ABSTRACT

In parts of Africa and Asia, self-medication with a hot water infusion of Artemisia annua (Artemisia tea) is a common practice for a number of ailments including malaria and cancer. In our earlier work, such an extract showed better potency than artemisinin alone against both chloroquine-sensitive and -resistant parasites. In this study, in vitro tests of the infusion in MCF7 cells showed high IC50 values (>200 μM). The combination of artemisinin and 3-caffeoylquinic acid (3CA), two major components in the extract, was strongly antagonistic and gave a near total loss of cytotoxicity for artemisinin. We observed that the interaction of 3CAs with another cytotoxic compound, cisplatin, showed potentiation of activity by 2.5-fold. The chelation of cellular iron by 3CA is hypothesized as a possible explanation for the loss of artemisinin activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium phosphate dibasic solution, BioUltra, 0.5 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium phosphate monobasic-16O4, 99.9 atom % 16O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
SKU
Pack Size
Availability
Price
Quantity
Supelco
Phosphate Standard for IC, TraceCERT®, 1000 mg/L phosphate in water (nominal concentration)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, ≥99.0% (titration)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, BioXtra, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
SKU
Pack Size
Availability
Price
Quantity
Supelco
Acetic acid, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sulforhodamine B, Dye content 75 %
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Trichloroacetic acid, BioUltra, ≥99.5% (T)
SKU
Pack Size
Availability
Price
Quantity
Cisplatin impurity A, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
cis-Diamineplatinum(II) dichloride, ≥99.9% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid solution, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss., 99-100%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
SKU
Pack Size
Availability
Price
Quantity
USP
Transplatin, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
Acetonitrile, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium phosphate dibasic, purum p.a., anhydrous, ≥98.0% (T)
SKU
Pack Size
Availability
Price
Quantity