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  • Voluntary feed intake in rainbow trout is regulated by diet-induced differences in oxygen use.

Voluntary feed intake in rainbow trout is regulated by diet-induced differences in oxygen use.

The Journal of nutrition (2013-04-26)
Subramanian Saravanan, Inge Geurden, A Cláudia Figueiredo-Silva, Sadasivam Kaushik, Johan Verreth, Johan W Schrama
ABSTRACT

This study investigated the hypothesis that the voluntary feed intake in fish is regulated by diet-induced differences in oxygen use. Four diets were prepared with a similar digestible protein:digestible energy ratio (18 mg/kJ), but which differed in the composition of nonprotein energy source. This replacement of fat (F) by starch (S) was intended to create a diet-induced difference in oxygen use (per unit of feed): diets F30-S70, F50-S50, F65-S35, and F80-S20 with digestible fat providing 28, 49, 65, and 81% of the nonprotein digestible energy (NPDE), respectively. Each diet was fed to satiation to triplicate groups of 20 rainbow trout for 6 wk. As expected, diet-induced oxygen use decreased linearly (R(2) = 0.89; P < 0.001) with increasing NPDE as fat. The digestible and metabolizable energy intakes of trout slightly increased with increasing NPDE as fat (i.e., decreasing starch content) (R(2) = 0.30, P = 0.08; and R(2) = 0.34, P = 0.05, respectively). Oxygen consumption of trout fed to satiation declined with increasing dietary NPDE as fat (R(2) = 0.48; P = 0.01). The inverse relation between digestible energy intake of trout and the diet-induced oxygen use (R(2) = 0.33; P = 0.05) suggests a possible role of diet-induced oxygen use in feed intake regulation as shown by the replacement of dietary fat by starch.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Starch from potato, suitable for electrophoresis
Sigma-Aldrich
Starch from wheat, Unmodified
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Starch from potato, Soluble
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Starch from potato, Powder
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Starch, puriss. p.a., from potato, reag. ISO, reag. Ph. Eur., soluble
Sigma-Aldrich
Starch, from potato, tested according to Ph. Eur.
Supelco
Starch from corn, analytical standard, analytical standard for Starch Assay Kits SA-20 and STA-20
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Starch from corn
Supelco
Starch from corn, for use with Total Dietary Fiber Control Kit, TDF-C10
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Starch from corn, practical grade
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Starch from rice
Sigma-Aldrich
Starch from corn, Unmodified waxy corn starch of essentially pure amylopectin; contains only trace amounts of amylose.