[Specific features of the anticonvulsant effect of memantine in mice intoxicated with a model organic phosphate].

[Specific features of the anticonvulsant effect of memantine in mice intoxicated with a model organic phosphate].

Eksperimental'naia i klinicheskaia farmakologiia (2013-05-02)

M A Iudin, S V Chepur, V N Bykov, A F Kurpiakova, S N Subbotina, A S Nikiforov, I M Ivanov

ABSTRACT

The effect of memantine administration has been studied on the model of mice poisoning with an anticholinesterase compound. It is established that the memantine action is due to its influence on the cholinesterase activity in the brain, blood plasma, and erythrocytes in addition to its NMDA-blocking action. Memantine promotes oxime-induced erythrocyte enzyme reactivation on the model of mice poisoning with anticholinesterase compound (0.8 LD50).

MATERIALS

Product Number

Brand

Product Description

C1682

Sigma-Aldrich

Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (Lowry)

D0879

Sigma-Aldrich

Diisopropylfluorophosphate

C3389

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel), Type VI-S, lyophilized powder, 200-1,000 units/mg protein

M3262

Sigma-Aldrich

N-Methyl-D-aspartic acid, ≥98% (TLC), solid

C0663

Sigma-Aldrich

Acetylcholinesterase from human erythrocytes, buffered aqueous solution, ≥500 units/mg protein (BCA)

C2888

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel), Type V-S, lyophilized powder, ≥1,000 units/mg protein