- An acyloxymethyl ketone-based probe to monitor the activity of glutathionylspermidine amidase in Escherichia coli.
An acyloxymethyl ketone-based probe to monitor the activity of glutathionylspermidine amidase in Escherichia coli.
Chembiochem : a European journal of chemical biology (2011-09-03)
Cheong-Meng Chong, Shijay Gao, Bing-Yu Chiang, Wen-Hung Hsu, Ting-Chien Lin, Tzu-Chieh Chen, Chun-Hung Lin
PMID21887840
ABSTRACT
Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Amidase from Pseudomonas aeruginosa, recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)