MTOX1012
CAR Knockout HepaRG™ Cells
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
Synonym(s):
CAR knockout human hepatocyte cells, HepaRG CAR KO
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.81
Recommended Products
product name
CAR Knockout HepaRG™ Cells, 1 vial
biological source
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
form
liquid
storage temp.
−196°C
Gene Information
human ... ABCB1(1346307)
General description
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG CAR KO cells express the major xenobiotic sensors (PXR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
Application
See technical bulletin for detailed protocols
Features and Benefits
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.
- Sigma′s HepaRG CAR Knockout (KO) allows conclusive identification of CYP2B6 induction through CAR activation.
Zinc finger nucleases (ZFN) mediated Knock-out of NR1I3 (CAR) gene.
- The frame-shift mutation of NR1I3 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.
Quality
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation
Legal Information
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daochuan Li et al.
Biochemical pharmacology, 98(1), 190-202 (2015-08-16)
The constitutive androstane receptor (CAR) modulates the transcription of numerous genes involving drug metabolism, energy homeostasis, and cell proliferation. Most functions of CAR however were defined from animal studies. Given the known species difference of CAR and the significant cross-talk
Wenwei Lin et al.
Molecular pharmacology, 97(3), 180-190 (2019-12-29)
The xenobiotic receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are activated by structurally diverse chemicals to regulate the expression of target genes, and they have overlapping regulation in terms of ligands and target genes. Receptor-selective agonists are
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