05-1049
Anti-Met (extracellular) Antibody, clone 4F8.2
clone 4F8.2, from mouse
Synonym(s):
HGF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, SF receptor, Scatter factor receptor, met proto-oncogene, met proto-oncogene (hepatocyte growth factor receptor), oncogene MET
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4F8.2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MET(4233)
Related Categories
General description
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
Specificity
Detects Met on its extracellular domain.
Immunogen
Epitope: Extracelluar domain (N-term)
GST-fusion protein corresponding to amino acids 80-434 of human Met.
Application
Detect Met (extracellular) using this Anti-Met (extracellular) Antibody, clone 4F8.2 validated for use in IH(P) & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Immunohistochemistry Analysis :
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Immunohistochemistry Analysis :
Quality
Routinely evaluated by Western Blot.
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
Target description
170 kDa
Linkage
Replaces: 05-237
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Charles Finsterwald et al.
The European journal of neuroscience, 34(7), 1053-1061 (2011-09-08)
Acquisition of a mature dendritic morphology is critical for neural information processing. In particular, hepatocyte growth factor (HGF) controls dendritic arborization during brain development. However, the cellular mechanisms underlying the effects of HGF on dendritic growth remain elusive. Here, we
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Nolan L Boyd et al.
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