Anodic TiO2 nanotubular arrays with pre-synthesized hydroxyapatite--an effective approach to enhance the biocompatibility of titanium.

Anodic TiO2 nanotubular arrays with pre-synthesized hydroxyapatite--an effective approach to enhance the biocompatibility of titanium.

Journal of nanoscience and nanotechnology (2013-07-26)

Lu-Ning Wang, Long-Xiang Lin, Chang-Jian Lin, Chen Shen, Alyssa Shinbine, Jing-Li Luo

ABSTRACT

Electrochemically anodized TiO2 nanotubular arrays can provide large surface areas for biological species attachment. In order to further enhance the biocompatibility of Ti medical implants, we deposited a pre-synthesized hydroxyapatite inside and on the nanotubular arrays, and examined the biocompatibility of the anodized TiO2 nanotubular arrays with pre-synthesized hydroxyapatite by in vitro assessment in simulated body fluid, and in vitro cell culture. The results showed that the hydroxyapatite coating was able to be induced on TiO2 nanotubular arrays with pre-synthesized hydroxyapatite within 5 days while only a thin film composed of calcium phosphorous chemicals formed on as-formed TiO2 nanotubular arrays. The cell culture evaluation further proved the enhancement of cell attachment and proliferation on TiO2 nanotubular arrays with pre-synthesized hydroxyapatite as opposed to those without pre-synthesized hydroxyapatite. The present study proves that formation of TiO2 nanotubular arrays with pre-synthesized hydroxyapatite a promising method to enhance the biocompatibility of Ti implants.

MATERIALS

Product Number

Brand

Product Description

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

634662

Sigma-Aldrich

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637262

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700347

Sigma-Aldrich

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21223

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Titanium, IRMM^®, certified reference material, 0.5 mm wire

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

266035

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204730

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348848

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224227

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Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

305812

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Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

267902

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Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

718467

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248576

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Sigma-Aldrich

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268496

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