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MIDCLARET

Sigma-Aldrich

CellVue® Claret Far Red Fluorescent Cell Linker Midi Kit for General Membrane Labeling

Distributed for Phanos Technologies

Synonym(s):

Far red membrane probe kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

technique(s)

flow cytometry: suitable

fluorescence

λex 655 nm; λem 675 nm

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

Related Categories

Application

CellVue Claret kit fluoresces in the far red and may be used for general cell membrane labeling including in vitro cell labeling, in vitro lymphocyte proliferation studies,† in vivo cytotoxicity assays,† and also as a second color for monitoring cell-cell membrane transfer.†

Biochem/physiol Actions

The CellVue Claret Fluorescent Cell Linker kit uses a proprietary membrane labeling technology to stably incorporate a far red fluorescent dye with long aliphatic tails (CellVue Claret) into lipid regions of the cell membrane.†

Packaging

This kit provides enough dye for up to 50 samples of 20 million cells each and enough diluent for up to 30 samples of 20 million cells each when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Linkage

For additional technical details on Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit PKH and CellVue®; Claret Fluorescent Cell Linker Dyes.

Legal Information

Distributed for Phanos Technologies, Inc.
Patent Information
CellVue is a registered trademark of Phanos Technologies

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 1

Certificates of Analysis (COA)

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Mercedes Rodriguez Garcia et al.
The Journal of clinical investigation, 120(7), 2486-2496 (2010-06-17)
One of the main unresolved questions in solid organ transplantation is how to establish indefinite graft survival that is free from long-term treatment with immunosuppressive drugs and chronic rejection (i.e., the establishment of tolerance). The failure to achieve this goal
Ryan W S Peacock et al.
Biotechnology and bioengineering, 108(9), 2003-2010 (2011-04-16)
In higher eukaryotes, E2F transcription factors often drive expression of genes necessary for the cell cycle, notably the G1/S phase transition. With conventional transcriptional reporter systems, expression of a reporter gene from an E2F-responsive promoter would allow one to identify
Allistair A Abraham et al.
Pediatric blood & cancer, 66(5), e27601-e27601 (2019-01-05)
Sickle cell disease (SCD) is increasingly recognized as a red blood cell disorder modulated by abnormally increased inflammation. We have previously shown that in patients with SCD not on a disease-modifying therapy (hydroxyurea or chronic transfusions), natural killer (NK) cell
Christopher Schliehe et al.
Journal of immunology (Baltimore, Md. : 1950), 187(5), 2112-2121 (2011-07-29)
The analysis of cell types involved in cross-priming of particulate Ag is essential to understand and improve immunotherapies using microparticles. In this study, we show that murine splenic dendritic cells (DCs) as well as macrophages (MΦs) are able to efficiently
ZAP70 expression directly promotes chronic lymphocytic leukaemia cell adhesion to bone marrow stromal cells.
Sandrine T Lafarge et al.
British journal of haematology, 168(1), 139-142 (2014-08-05)
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