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Key Documents

P0532

Sigma-Aldrich

Phthaldialdehyde Reagent

Solution Complete

Synonym(s):

OPA

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21

Quality Level

storage temp.

2-8°C

SMILES string

[H]C(=O)c1ccccc1C([H])=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H

InChI key

ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Application

Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.

Other Notes

Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.

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Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1

WGK

WGK 3


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Silke B Lohan et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-25)
The daily consumption of tobacco products leads to a boost in free radical production in tissues, promoting the risk for malignancies, metabolic alterations and chronic-inflammatory diseases. This study aimed to broaden the knowledge of the status of the antioxidative (AO)
Tomáš Roušar et al.
Analytical biochemistry, 423(2), 236-240 (2012-03-06)
Glutathione is an important tripeptide involved in a variety of cellular processes. Thus, precise knowledge of its levels is essential. Glutathione exists in two free forms-reduced and oxidized-and a number of methods exist to measure its levels. The aim of
Jianhua Liu et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 91, 307-313 (2012-03-06)
Relying on the reaction of o-phthalaldehyde (OPA) with glutathione (GSH) to form a highly fluorescence derivative GSH-OPA has been widely used to measure reduced glutathione. In order to better understand spectra property of the GSH-OPA and the effect of zinc
M Nurul Islam et al.
The New phytologist, 193(1), 51-57 (2011-11-11)
• Sphingolipids are emerging as important mediators of cellular and developmental processes in plants, and advances in lipidomics have yielded a wealth of information on the composition of plant sphingolipidomes. Studies using Arabidopsis thaliana showed that the dihydroxy long-chain base
Quansong Li et al.
Physical chemistry chemical physics : PCCP, 14(18), 6561-6568 (2012-03-30)
The potential energy surface for the intramolecular excited state hydrogen transfer (IESHT) in ortho-phthalaldehyde (OPA), which generates an enol ketene, has been studied with ab initio calculations (MS-CASPT2//CASSCF). The goal of our study is to establish the mechanistic factors that

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