MABN712
Anti-CCL2 Antibody, clone 2D8
clone 2D8, from mouse
Synonym(s):
C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D8, monoclonal
species reactivity
monkey, mouse, rat, human
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CCL2(6347)
General description
CCL2, Chemokine (C-C motif) ligand 2, or alternatively known as Monocyte chemotactic protein 1 (MCP-1), Monocyte chemotactic and activating factor (MCAF), Monocyte chemoattractant protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, HC11, and C-C motif chemokine 2 is encoded by the human gene named CCL2/MCP1/SCYA2. CCL2 is a chemokine produced by endothelial cells that attracts memory T cells, monocytes, dendritic cells, and basophils but not neutrophils or eosinophils to sites of inflammation produced by either tissue inquiry or infection. CCL2 also bolsters monocytes anti-tumor activity, and because it is chemotactic for monocytes, CCL2 has been implicated in inflammatory diseases where monocytic invasions have occurred such as in psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is a bifunctional molecule as well; the whole molecule is a basophil activator but removal of the N-terminal portion converts the molecule into an exclusive eosinophil chemoattractant. CCL2 can also be found expressed at the sites of tooth eruption and bone degradation and is expressed by both osteoclasts and osteoblasts. CCL2 is also expressed in neurons, astrocytes and microglia and its role in neuroinflammation is established. CCL2 expression in glial and neurons is increased in epilepsy, brain ischemia, Alzheimer’s disease, brain injury and experimental autoimmune encephalomyelitis. Finally, CCL2 plays a role in vascular complications associated with type II diabetes and in the production of amylin which contributes to insulin resistance in obesity, which is also associated with type II diabetes. EMD-Millipore’s Anti-CCL2 mouse monoclonal antibody has been tested in western blot against recombinant protein and cell lysates from A549, HeLa, Raw264.7, L1210, C6, and COS7 cell lines.
Immunogen
Purified recombinant fragment of human CCL2 expressed in E. Coli.
Application
Detect Monocyte Chemotactic Protein using this mouse monoclonal antibody, Anti-CCL2 Antibody, clone 2D8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in HeLa cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CCL2 in human liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CCL2 in A549 cells.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Quality
Evaluated by Western Blotting in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CCL2 in A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Target description
~14 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Format: Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
A549, HeLa, Raw264.7, L1210, C6 and COS-7 cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lisha Luo et al.
Thoracic cancer, 13(6), 811-823 (2022-02-10)
Monocytes are involved in tumor growth and metastasis, but the distribution of monocyte phenotypes and their role in the development of malignant pleural effusion (MPE) remains unknown. A total of 94 MPE patients (76 diagnosed with adenocarcinoma lung cancer and
Victoria Macht et al.
Journal of neuroinflammation, 18(1), 212-212 (2021-09-18)
Binge ethanol exposure during adolescence reduces hippocampal neurogenesis, a reduction which persists throughout adulthood despite abstinence. This loss of neurogenesis, indicated by reduced doublecortin+ immunoreactivity (DCX+IR), is paralleled by an increase in hippocampal proinflammatory signaling cascades. As galantamine, a cholinesterase
Xue Shi et al.
BMC neuroscience, 23(1), 20-20 (2022-04-01)
C-C motif chemokine ligand 2 (CCL2) is reported to be involved in the pathogenesis of various neurological and/or psychiatric diseases. Tissue or cellular expression of CCL2, in normal or pathological condition, may play an essential role in recruiting monocytes or
Milene Volpato et al.
Oncotarget, 7(19), 28139-28150 (2016-04-09)
The mechanism of the anti-colorectal cancer (CRC) activity of the omega-3 fatty acid eicosapentaenoic acid (EPA) is not understood. We tested the hypothesis that EPA reduces expression of chemokine C-C motif ligand 2 (CCL2), a pro-inflammatory chemokine with known roles
Aya Misawa et al.
Genes, 12(2) (2021-01-31)
Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression, which play fundamental roles in cancer development. In this study, we found that homeobox A11 antisense RNA (HOXA11-AS), a highly expressed lncRNA in cell lines derived from prostate
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