biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7G6, monoclonal
mol wt
calculated mol wt 42.67 kDa
purified by
using protein G
species reactivity
human, monkey, bovine
packaging
antibody small pack of 100 μg
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... arr3> ARR3(102134564)
General description
Arrestin-C (UniProt: A0A2K5U9A7; also known as Cone arrestin, Retinal cone arrestin-3) is encoded by the ARR3 gene (Gene ID: 102134564) in Monkey. Arrestins are a superfamily of multi-functional proteins that that regulate signaling and trafficking of the majority of G-protein-coupled receptors (GPCRs), as well as sub-cellular localization and activity of many other signaling proteins. Arrestin-C is predominantly found in inner and outer segments, and the inner plexiform regions of the retina. It is expressed in cone photoreceptors and pinealocytes and may contribute to the shut-off mechanisms associated with high acuity color vision. Arrestin-C is an elongated two-domain molecule with overall fold and key inter-domain interactions that hold the free protein in the basal conformation similar to the other subtypes. Several structural elements are reported to contribute to arrestin binding. The C-terminal acidic region serves a regulatory role in controlling arrestin binding selectivity toward the phosphorylated and activated form of a receptor. The basic N-terminal domain directly participates in receptor interaction and serves a regulatory role via intramolecular interaction with the C-terminal acidic region. Also, two centrally localized domains are directly involved in determining receptor binding specificity and selectivity. Clone 7G6 is a cone-specific marker that recognizes all cones in the adult primate retina. In fetal retina, although long and middle wavelength-sensitive cones recognized by clone 7G6, a subset of short wavelength-sensitive cones are shown to be delayed in their reactivity to this clone. (Ref.: Zhang, H., et al. (2003). Invest. Opthalmol. Vis. Sci. 44(7); 2858-2867; Wikler, KC., et al. (1997). J. Comp. Neurol. 377(4); 500-508).
Specificity
Clone 7G6 is a mouse monoclonal antibody that detects cone arrestin (Arrestin-C). It targets an epitope within the N-terminal region.
Immunogen
Crude retinal extract from Monkey (Macaca fascicularis).
Application
Quality Control Testing
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Tested Applications
Western Blotting Analysis: A representative lot detected Arrestin-C in Western Blotting applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).
Immunohistochemistry Applications: A representative lot detected Arrestin-C in Immunohistochemistry applications (Wikler, K.C., et al. (1997). J Comp Neurol.;377(4):500-8; John, S.K., et al. (2000). Mol Vis.;6:204-15; Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67; O′Brien, J.J., et al. (2012). J Neurosci.;32(13):4675-87).
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in frozen monkey retina tissues (Courtesy of Prof. Peter MacLeish, Ph.D., photo by Talib Saafir, Ph.D., Morehouse School of Medicine, Atlanta, GA, USA).
Immunofluorescence Analysis: A representative lot detected Arrestin-C in Immunofluorescence applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Tested Applications
Western Blotting Analysis: A representative lot detected Arrestin-C in Western Blotting applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).
Immunohistochemistry Applications: A representative lot detected Arrestin-C in Immunohistochemistry applications (Wikler, K.C., et al. (1997). J Comp Neurol.;377(4):500-8; John, S.K., et al. (2000). Mol Vis.;6:204-15; Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67; O′Brien, J.J., et al. (2012). J Neurosci.;32(13):4675-87).
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Arrestin-C in frozen monkey retina tissues (Courtesy of Prof. Peter MacLeish, Ph.D., photo by Talib Saafir, Ph.D., Morehouse School of Medicine, Atlanta, GA, USA).
Immunofluorescence Analysis: A representative lot detected Arrestin-C in Immunofluorescence applications (Zhang, H., et al. (2003). Invest Ophthalmol Vis Sci.;44(7):2858-67).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Arrestin-C, clone 7G6, Cat. No. MABN2636, is a mouse monoclonal antibody that detects Arrestin-C and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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