1.10405

GranuCult^® plus Letheen-Broth Base modified

FDA-BAM, suitable for microbiology

• Synonym(s): Letheen Broth, Letheen Modified Broth, Modified Letheen Broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: FDA-BAM
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• pH: 7.0-7.4 (25 °C, 37.8 g/L in H₂O, after autoclaving)
• solubility: 37.8 g/L
• application(s): cosmetics; microbiology
• storage temp.: 15-25°C
• suitability: Enterococcus spp.; Escherichia coli; Pseudomonas spp.; Salmonella spp.; Staphylococcus spp.

Description

General description

GranuCult^® plus Letheen-Broth Base modified is highly nutritious containing Lecithin and Tween^® 80 for neutralizing quaternary ammonium compounds. Beef extract and tryptone provide nitrogen, carbon, sulfur, and other essential growth factors. Glucose is the source of energy. These media are modifications of the AOAC formulae. Letheen Broth Base is used for determining the phenol coefficient of quaternary compounds.

Application

GranuCult^® plus Letheen-Broth Base modified is a unique formulation for determining the bacterial activity of quaternary ammonium compounds.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-lowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): turbid
Appearance (colour): brown
pH-value (25 °C): 7.0 - 7.4
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): good to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Incubation: 24 to 48 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3